Wilms_Tumour_organoid_sequencing_WGS

This study investigates the genomic and transcriptomic characteristics of Wilm's tumour organoids

Type: Cancer Genomics
Archiver: European Genome-Phenome Archive (EGA)

3 Datasets 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD00001005312	This study investigates the genomic and transcriptomic characteristics of Wilm's tumour organoids . This dataset contains all the data available for this study on 2019-09-05.	HiSeq X Ten	-
EGAD00001009812	Cancers of adults typically arise through progressive rounds of clonal diversification and intratumoral selective sweeps which generate a long mutational trunk with shorter subclonal branches. Here, we investigated whether tumors of young children exhibit the same phylogenetic configuration. We studied three infants, including two newborns, with the childhood kidney cancer, Wilms tumour, through whole genome sequencing of bulk tissues, of single cell derived organoids, and of microdissections. All three cancers exhibited unusual driver events, with tumours of newborns harbouring FOXR2 rearrangements, delineating a distinct variant of Wilms tumour. Phylogenetic analyses suggest that tumors were seeded in an early, possibly confined window of development. Unusually, following seeding there was extensive polyclonal diversification with little evidence of clonal sweeps, leading to a distinct phylogenetic configuration more reminiscent of normal tissues rather than of adult cancers. These findings indicate that some childhood cancers may diversify via unorthodox phylogenetic pathways.	HiSeq X Ten Illumina NovaSeq 6000	-
EGAD00001015406	A paradigm of childhood cancers is that they have a low mutation burden, with some ostensibly bearing fewer mutations than the normal tissues from which they derive. We set out to resolve this paradox by examining paediatric renal cancers with exceptionally few mutations using high resolution, high depth sequencing approaches. We found that apparent hypomutation was the result of unusual clonal architecture due to a normal tissue-like mode of tumour evolution, raising the possibility that the mutation burden of some cancers has been systematically misjudged.	HiSeq X Ten Illumina NovaSeq 6000	-

Publications	Citations
High resolution clonal architecture of hypomutated Wilms tumours. Lee-Six H, Treger TD, Dave M, Coorens TH, Anderson ND, Tiersma Y, Derakhshan S, de Haan S, van den Heuvel-Eibrink MM, Wang Y, Wenger A, Al-Saadi R, Lawford A, Letunovska A, Wegert J, Parks C, Morcrette G, Gessler M, Vujanic G, Chowdhury T, J O'Sullivan M, de Krijger RR, Stratton MR, Pritchard-Jones K, Hutchinson JC, Drost J, Behjati S. Nat Commun 16: 2025 4647	2

Publications

Citations

High resolution clonal architecture of hypomutated Wilms tumours.
Lee-Six H, Treger TD, Dave M, Coorens TH, Anderson ND, Tiersma Y, Derakhshan S, de Haan S, van den Heuvel-Eibrink MM, Wang Y, Wenger A, Al-Saadi R, Lawford A, Letunovska A, Wegert J, Parks C, Morcrette G, Gessler M, Vujanic G, Chowdhury T, J O'Sullivan M, de Krijger RR, Stratton MR, Pritchard-Jones K, Hutchinson JC, Drost J, Behjati S. Nat Commun 16: 2025 4647