Exome and RNA sequencing of Greenlanders

Study ID Alternative Stable ID Type
EGAS00001002727 Other

Study Description

This study contains whole exome sequence of 27 Greenlanders and deep RNA sequencing of 17 Greenlanders

Study Datasets 2 datasets.

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Dataset ID Description Technology Samples
The data contain whole exome sequencing of 27 Greenlanders in nine trios. Data were produced by Agilent SureSelect capture followed by paired-end Illumina HiSeq 2000 sequencing to a depth of 90.1X. More details on processing and analysis can be found in Moltke et al, Nature 2014 (PMID 25043022).
Illumina HiSeq 2000 27
The data contain whole deep RNA sequencing of leukocytes from 17 Greenlanders. RNA was purified from peripheral blood with the PAXGene Blood miRNA Kit (Qiagen). The RNA sequencing library was prepared following the instructions of the TruSeq RNA Sample Prep Kit v2 (Illumina). For mRNA isolation and fragmentation 200 ng of total RNA was purified by oligo-dT beads. The qualified libraries were amplified on cBot to generate the cluster on the flowcell (TruSeq PE Cluster Kit V3–cBot–HS, ... (Show More)
Illumina HiSeq 4000 17

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