RNA sequencing of peripheral blood samples from 17 Greenlanders

Dataset ID Technology Samples
EGAD00001003814 Illumina HiSeq 4000 17

Dataset Description

The data contain whole deep RNA sequencing of leukocytes from 17 Greenlanders. RNA was purified from peripheral blood with the PAXGene Blood miRNA Kit (Qiagen). The RNA sequencing library was prepared following the instructions of the TruSeq RNA Sample Prep Kit v2 (Illumina). For mRNA isolation and fragmentation 200 ng of total RNA was purified by oligo-dT beads. The qualified libraries were amplified on cBot to generate the cluster on the flowcell (TruSeq PE Cluster Kit V3–cBot–HS, Illumina). The amplified flow cell was sequenced paired-end on the HiSeq 4000 System (TruSeq SBS KIT-HS V3, Illumina).

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

DAC for Greenland Studies of University of Copenhagen and University of Southern Denmark.
Contact person: Christina Viskum Lytken Larsen
Email: cll [at] si-folkesundhed [dot] dk
More details: EGAC00001000736


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