RNA Sequencing of Colorectal Liver Metastases

A subset of patients with metastatic colorectal cancer achieves prolonged survival after hepatic resection of limited liver metastases. Here, we examined the molecular features of colorectal liver metastases as they relate to clinical outcomes.

Type: Other
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 3 Publications

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD00001004111	Reverse-stranded paired-end 75 base-pair RNA sequencing libraries of 93 metastatic FFPE samples were constructed using Illumina Total RNA Stranded Kits. Ribosomal RNAs (rRNAs) were depleted by using the Ribo-Zero rRNA Removal Kit (Illumina). Libraries were sequenced on a HiSEQ2500 machine. Five samples were re-sequenced using paired-end 50 base-pair libraries due to the smaller insert sizes.	Illumina HiSeq 2500	93

Publications	Citations
Integrated molecular subtyping defines a curable oligometastatic state in colorectal liver metastasis. Pitroda SP, Khodarev NN, Huang L, Uppal A, Wightman SC, Ganai S, Joseph N, Pitt J, Brown M, Forde M, Mangold K, Xue L, Weber C, Segal JP, Kadri S, Stack ME, Khan S, Paty P, Kaul K, Andrade J, White KP, Talamonti M, Posner MC, Hellman S, Weichselbaum RR. Nat Commun 9: 2018 1793	118
Colorectal Cancer Metastases in the Liver Establish Immunosuppressive Spatial Networking between Tumor-Associated SPP1+ Macrophages and Fibroblasts. Sathe A, Mason K, Grimes SM, Zhou Z, Lau BT, Bai X, Su A, Tan X, Lee H, Suarez CJ, Nguyen Q, Poultsides G, Zhang NR, Ji HP. Clin Cancer Res 29: 2023 244-260	46
A proliferative subtype of colorectal liver metastases exhibits hypersensitivity to cytotoxic chemotherapy. Spurr LF, Martinez CA, Katipally RR, Iyer SC, Pugh SA, Bridgewater JA, Primrose JN, Domingo E, Maughan TS, D'Angelica MI, Talamonti M, Posner MC, Connell PP, Weichselbaum RR, Pitroda SP. NPJ Precis Oncol 6: 2022 72	5