Study

Inference of B cell clonality and function from single cell RNA seq data

Study ID Alternative Stable ID Type
EGAS00001002963 Transcriptome Analysis

Study Description

cDNA libraries for RNA-seq will be prepared for sequencing. cDNA will be created following the Smart-Seq2 protocol, and a part of the cDNA from each cell will be used for preparation of RNA-seq libraries using the Nextera XT kit. The other part of the cDNA will be used as template for verification of the computationally recovered immunoglobulin sequences from the RNA-seq dataset by using a targeted PCR approach to generate a multiplexed sequencing library

Study Datasets 1 dataset.

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID Description Technology Samples
EGAD00001004199
This dataset was made to verify the computational reconstruction of B cell reseptors from single-cell RNA-seq using BraCeR. The dataset contains BCR-derived reads from single-cell RNA-seq from 13 cells using the Smart-seq2 protocol, as well as targeted BCR-sequencing data from the same cells.
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Who archives the data?

There are no publications available