FAM50A Disruption in TOV21G Cells RNAseq

Study ID Alternative Stable ID Type
EGAS00001004156 Cancer Genomics

Study Description

In this project we aimed to assess the transcriptional consequences of disrupting FAM50A in TOV21G cells in vivo. To do this we generated a dox inducible FAM50A gRNA allele such that we could conditionally inactivate FAM50A by feeding mice a diet containing doxycycline. Tumour masses were harvested from mice when they reached the ethical limit and RNA was extracted for RNA-Seq analysis.

Study Datasets 1 dataset.

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Dataset ID Description Technology Samples
Genetic redundancy has evolved as a way for human cells to survive the loss of genes that are single copy and essential in other organisms, but also allows tumours to survive despite having highly rearranged genomes. In this study we CRISPR screen 1,191 gene pairs, including paralogues and known and predicted synthetic lethal interactions to identify 105 gene combinations whose co-disruption results in a loss of cellular fitness. 27 pairs influence fitness across multiple cell lines including ... (Show More)
Illumina HiSeq 4000,Illumina NovaSeq 6000 55

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