RNA sequencing of chondrosarcoma

Study ID Alternative Stable ID Type
EGAS00001004585 Other

Study Description

To profile chondrosarcoma patients.

Study Datasets 1 dataset.

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Dataset ID Description Technology Samples
Transcriptome sequencing of nine patients diagnosed with chondrosarcoma. cDNA was generated using the NuGEN Ovation RNA-Seq FFPE system. Total RNA was randomly primed and thermally sheared and the resulting cDNA fragment was amplified. The cDNA was then mechanically sheared using sonication to generate ~250 bp fragments. Sequencing libraries were generated using the NuGEN Ovation Ultralow System V2 library prep kit and sequenced on HiSeq2500 TruSeq v3.
Illumina HiSeq 2500 8

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