Study
Single-cell proteo-genomic reference maps of the human hematopoietic system
| Study ID | Alternative Stable ID | Type |
|---|---|---|
| EGAS00001005593 | Other |
Study Description
We have generated high content single-cell proteo-genomic reference maps of human blood and bone marrow that quantitatively link the expression of up to 197 surface markers to cellular identities and biological processes across all major hematopoietic cell types in healthy aging and leukemia. Please note raw and integrated gene expression data, cell type annotation, metadata and dimensionality reduction are available as Seurat v3 objects through figshare. Access links are https://figshare.com/s/0fda29b169c719223ee3 (97 surface marker, 9 individuals dataset), https://figshare.com/s/313b5739ff469dac8c01 (197 surface marker, bone marrow and blood dataset), https://figshare.com/s/901bcddb9ee18e226031 (97 surface markers and whole transcriptome dataset) and https://figshare.com/s/24e10bf129fae1918316 (97 surface marker, 15 AML datasets).
Study Datasets 7 datasets.
Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data
| Dataset ID | Description | Technology | Samples |
|---|---|---|---|
| EGAD00001008184 |
Targeted sequencing using BD Rhapsody with 462 mRNA of healthy young adult bone marrow mononuclear cells from iliac crest aspirations (BM3/Young3).
|
Illumina HiSeq 4000 | 1 |
| EGAD00001008185 |
Shallow targeted sequencing with 462 mRNA and 97 antibodies of AML patient’s bone marrow mononuclear cells from iliac crest aspirations from. Please note raw and integrated gene expression data, cell type annotation, metadata and dimensionality reduction are available as Seurat v3 objects through figshare. Access link is ... (Show More)
|
Illumina NovaSeq 6000 | 1 |
| EGAD00001008186 |
Whole transcriptome sequencing using BD Rhapsody with 97 antibodies of a healthy young adult bone marrow (Young3/BM3) mononuclear cells from iliac crest aspirations. Please note raw and integrated gene expression data, cell type annotation, metadata and dimensionality reduction are available as Seurat v3 objects through figshare. Access links is https://figshare.com/s/901bcddb9ee18e226031.
|
Illumina HiSeq 4000 | 1 |
| EGAD00001008187 |
SmartSeq2 read out of index cultured cell sorted with the classification and erythroid-myeloid panel developed in main Figure 6.
|
Illumina HiSeq 4000 | 10 |
| EGAD00001008188 |
Targeted sequencing using BD Rhapsody with 462 mRNA and 97 antibodies of healthy young and aged adult as well as AML bone marrow mononuclear cells from iliac crest aspirations. Please note raw and integrated gene expression data, cell type annotation, metadata and dimensionality reduction are available as Seurat v3 objects through figshare. Access link is https://figshare.com/s/0fda29b169c719223ee3.
|
NextSeq 500 | 9 |
| EGAD00001008189 |
Targeted sequencing using BD Rhapsody with 462 mRNA and 197 antibodies of healthy young adult bone marrow mononuclear cells from iliac crest aspirations. Please note raw and integrated gene expression data, cell type annotation, metadata and dimensionality reduction are available as Seurat v3 objects through figshare. Access link is https://figshare.com/s/313b5739ff469dac8c01
|
Illumina HiSeq 4000 | 2 |
| EGAD00001008190 |
Targeted sequencing with 462 mRNA and 97 antibodies of fresh, frozen and stored on ice (6h) healthy adult blood cells. Multiplexed sample fresh thawed ice SMK1-Frozen, SMK2-thawed, SMK3-fresh and Targeted sequencing with 462 mRNA and 197 antibodies of CD34+Immature cells Multiplexed sample CD34+ immature Abseq SMK4-CD38+CD45RA-, SMK5-CD38+CD45RA+, SMK6-CD38-CD45RA+/-
|
Illumina NovaSeq 6000 | 1 |
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