Single-cell proteo-genomic reference maps of the human hematopoietic system

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD00001008184	Targeted sequencing using BD Rhapsody with 462 mRNA of healthy young adult bone marrow mononuclear cells from iliac crest aspirations (BM3/Young3).	Illumina HiSeq 4000	1
EGAD00001008185	Shallow targeted sequencing with 462 mRNA and 97 antibodies of AML patient’s bone marrow mononuclear cells from iliac crest aspirations from. Please note raw and integrated gene expression data, cell type annotation, metadata and dimensionality reduction are available as Seurat v3 objects through figshare. Access link is https://doi.org/10.6084/m9.figshare.14780127.v1 AMLQ4_SMK1 AML314 male AMLQ1_SMK2 AML116 female AMLQ3_SMK3 AML127 female AMLQ6_SMK4 AML183 male AMLQ2_SMK5 AML327 female AMLQ5_SMK6 AML334 male APLQ5_SMK7 APL124 male APLQ3_SMK8 APL142 male APLQ6_SMK9 APL218 female APLQ4_SMK10 APL147 male APLQ2_SMK11 APL223 female APLQ1_SMK12 APL224 female	Illumina NovaSeq 6000	1
EGAD00001008186	Whole transcriptome sequencing using BD Rhapsody with 97 antibodies of a healthy young adult bone marrow (Young3/BM3) mononuclear cells from iliac crest aspirations. Please note raw and integrated gene expression data, cell type annotation, metadata and dimensionality reduction are available as Seurat v3 objects through figshare. Access links is https://figshare.com/s/901bcddb9ee18e226031.	Illumina HiSeq 4000	1
EGAD00001008187	SmartSeq2 read out of index cultured cell sorted with the classification and erythroid-myeloid panel developed in main Figure 6.	Illumina HiSeq 4000	10
EGAD00001008188	Targeted sequencing using BD Rhapsody with 462 mRNA and 97 antibodies of healthy young and aged adult as well as AML bone marrow mononuclear cells from iliac crest aspirations. Please note raw and integrated gene expression data, cell type annotation, metadata and dimensionality reduction are available as Seurat v3 objects through figshare. Access link is https://figshare.com/s/0fda29b169c719223ee3.	NextSeq 500	9
EGAD00001008189	Targeted sequencing using BD Rhapsody with 462 mRNA and 197 antibodies of healthy young adult bone marrow mononuclear cells from iliac crest aspirations. Please note raw and integrated gene expression data, cell type annotation, metadata and dimensionality reduction are available as Seurat v3 objects through figshare. Access link is https://figshare.com/s/313b5739ff469dac8c01	Illumina HiSeq 4000	2
EGAD00001008190	Targeted sequencing with 462 mRNA and 97 antibodies of fresh, frozen and stored on ice (6h) healthy adult blood cells. Multiplexed sample fresh thawed ice SMK1-Frozen, SMK2-thawed, SMK3-fresh and Targeted sequencing with 462 mRNA and 197 antibodies of CD34+Immature cells Multiplexed sample CD34+ immature Abseq SMK4-CD38+CD45RA-, SMK5-CD38+CD45RA+, SMK6-CD38-CD45RA+/-	Illumina NovaSeq 6000	1

Publications	Citations
Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states. Triana S, Vonficht D, Jopp-Saile L, Raffel S, Lutz R, Leonce D, Antes M, Hernández-Malmierca P, Ordoñez-Rueda D, Ramasz B, Boch T, Jann JC, Nowak D, Hofmann WK, Müller-Tidow C, Hübschmann D, Alexandrov T, Benes V, Trumpp A, Paulsen M, Velten L, Haas S. Nat Immunol 22: 2021 1577-1589	44
Selective homing of CAR-CIK cells to the bone marrow niche enhances control of the acute myeloid leukemia burden. Biondi M, Tettamanti S, Galimberti S, Cerina B, Tomasoni C, Piazza R, Donsante S, Bido S, Perriello VM, Broccoli V, Doni A, Dazzi F, Mantovani A, Dotti G, Biondi A, Pievani A, Serafini M. Blood 141: 2023 2587-2598	6

Publications

Citations

Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states.
Triana S, Vonficht D, Jopp-Saile L, Raffel S, Lutz R, Leonce D, Antes M, Hernández-Malmierca P, Ordoñez-Rueda D, Ramasz B, Boch T, Jann JC, Nowak D, Hofmann WK, Müller-Tidow C, Hübschmann D, Alexandrov T, Benes V, Trumpp A, Paulsen M, Velten L, Haas S. Nat Immunol 22: 2021 1577-1589

Selective homing of CAR-CIK cells to the bone marrow niche enhances control of the acute myeloid leukemia burden.
Biondi M, Tettamanti S, Galimberti S, Cerina B, Tomasoni C, Piazza R, Donsante S, Bido S, Perriello VM, Broccoli V, Doni A, Dazzi F, Mantovani A, Dotti G, Biondi A, Pievani A, Serafini M. Blood 141: 2023 2587-2598