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Targeted sequencing of healthy individuals, aged individuals and AML patients

Targeted sequencing using BD Rhapsody with 462 mRNA and 97 antibodies of healthy young and aged adult as well as AML bone marrow mononuclear cells from iliac crest aspirations. Please note raw and integrated gene expression data, cell type annotation, metadata and dimensionality reduction are available as Seurat v3 objects through figshare. Access link is https://figshare.com/s/0fda29b169c719223ee3.

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Single-cell proteo-genomic reference map data access policy

DATA ACCESS AGREEMENT These terms and conditions govern access to the managed access datasets (details of which are set out in Appendix I) to which the User Institution has requested access. The User Institution agrees to be bound by these terms and conditions. Definitions Authorised Personnel: The individuals at the User Institution to whom Single-cell proteo-genomic reference map DAC grants access to the Data. This includes the User, the individuals listed in Appendix II and any other individuals for whom the User Institution subsequently requests access to the Data. Details of the initial Authorised Personnel are set out in Appendix II. Data: The managed access datasets to which the User Institution has requested access. Data Producers: Single-cell proteo-genomic reference map DAC and the collaborators listed in Appendix I responsible for the development, organisation, and oversight of these Data. External Collaborator: A collaborator of the User, working for an institution other than the User Institution. Project: The project for which the User Institution has requested access to these Data. A description of the Project is set out in Appendix II. Publications: Includes, without limitation, articles published in print journals, electronic journals, reviews, books, posters and other written and verbal presentations of research. Research Participant: An individual whose data form part of these Data. Research Purposes: Shall mean research that is seeking to advance the understanding of genetics and genomics, including the treatment of disorders, and work on statistical methods that may be applied to such research. User: The principal investigator for the Project. User Institution(s): The Institution that has requested access to the Data. User Institution(s): The Institution that has requested access to the Data. 1. The User Institution agrees to only use these Data for the purpose of the Project (described in Appendix II) and only for Research Purposes. The User Institution further agrees that it will only use these Data for Research Purposes which are within the limitations (if any) set out in Appendix I. 2. The User Institution agrees to preserve, at all times, the confidentiality of these Data. In particular, it undertakes not to use, or attempt to use these Data to compromise or otherwise infringe the confidentiality of information on Research Participants. Without prejudice to the generality of the foregoing, the User Institution agrees to use at least the measures set out in Appendix I to protect these Data. 3. The User Institution agrees to protect the confidentiality of Research Participants in any research papers or publications that they prepare by taking all reasonable care to limit the possibility of identification. 4. The User Institution agrees not to link or combine these Data to other information or archived data available in a way that could re-identify the Research Participants, even if access to that data has been formally granted to the User Institution or is freely available without restriction. 5. The User Institution agrees only to transfer or disclose these Data, in whole or part, or any material derived from these Data, to the Authorised Personnel. Should the User Institution wish to share these Data with an External Collaborator, the External Collaborator must complete a separate application for access to these Data. 6. The User Institution agrees that the Data Producers, and all other parties involved in the creation, funding or protection of these Data: a) make no warranty or representation, express or implied as to the accuracy, quality or comprehensiveness of these Data; b) exclude to the fullest extent permitted by law all liability for actions, claims, proceedings, demands, losses (including but not limited to loss of profit), costs, awards damages and payments made by the Recipient that may arise (whether directly or indirectly) in any way whatsoever from the Recipient’s use of these Data or from the unavailability of, or break in access to, these Data for whatever reason and; c) bear no responsibility for the further analysis or interpretation of these Data. 7. The User Institution agrees to follow the Fort Lauderdale Guidelines (http://www.wellcome.ac.uk/stellent/groups/corporatesite/@policy_communications/documents/web_document/wtd003207.pdf ) and the Toronto Statement (http://www.nature.com/nature/journal/v461/n7261/full/461168a.html). This includes but is not limited to recognising the contribution of the Data Producers and including a proper acknowledgement in all reports or publications resulting from the use of these Data. 8. The User Institution agrees to follow the Publication Policy in Appendix III. This includes respecting the moratorium period for the Data Producers to publish the first peer-reviewed report describing and analysing these Data. 9. The User Institution agrees not to make intellectual property claims on these Data and not to use intellectual property protection in ways that would prevent or block access to, or use of, any element of these Data, or conclusion drawn directly from these Data. 10. The User Institution can elect to perform further research that would add intellectual and resource capital to these data and decide to obtain intellectual property rights on these downstream discoveries. In this case, the User Institution agrees to implement licensing policies that will not obstruct further research and to follow the U.S. National Institutes of Health Best Practices for the Licensing of Genomic Inventions (2005) (https://www.icgc.org/files/daco/NIH_BestPracticesLicensingGenomicInventions_2005_en.pdf ) in conformity with the Organisation for Economic Co-operation and Development Guidelines for the Licensing of the Genetic Inventions (2006) (http://www.oecd.org/science/biotech/36198812.pdf ). 11. The User Institution agrees to destroy/discard the Data held, once it is no longer used for the Project, unless obliged to retain the data for archival purposes in conformity with audit or legal requirements. 12. The User Institution will notify Single-cell proteo-genomic reference map DAC within 30 days of any changes or departures of Authorised Personnel. 13. The User Institution will notify Single-cell proteo-genomic reference map DAC prior to any significant changes to the protocol for the Project. 14. The User Institution will notify Single-cell proteo-genomic reference map DAC as soon as it becomes aware of a breach of the terms or conditions of this agreement. 15. Single-cell proteo-genomic reference map DAC may terminate this agreement by written notice to the User Institution. If this agreement terminates for any reason, the User Institution will be required to destroy any Data held, including copies and backup copies. This clause does not prevent the User Institution from retaining these data for archival purpose in conformity with audit or legal requirements. 16. The User Institution accepts that it may be necessary for the Data Producers to alter the terms of this agreement from time to time. As an example, this may include specific provisions relating to the Data required by Data Producers other than Single-cell proteo-genomic reference map DAC. In the event that changes are required, the Data Producers or their appointed agent will contact the User Institution to inform it of the changes and the User Institution may elect to accept the changes or terminate the agreement. 17. If requested, the User Institution will allow data security and management documentation to be inspected to verify that it is complying with the terms of this agreement. 18. The User Institution agrees to distribute a copy of these terms to the Authorised Personnel. The User Institution will procure that the Authorised Personnel comply with the terms of this agreement. 19. This agreement (and any dispute, controversy, proceedings or claim of whatever nature arising out of this agreement or its formation) shall be construed, interpreted and governed by the laws of England and Wales and shall be subject to the exclusive jurisdiction of the English courts. Agreed for User Institution Signature: Name: Title: Date: Principal Investigator I confirm that I have read and understood this Agreement. Signature: Name: Title: Date: Agreed for Single-cell proteo-genomic reference map DAC Signature: Name: Title: Date: APPENDIX I – DATASET DETAILS APPENDIX II ––PROJECT DETAILS APPENDIX III –– PUBLICATION POLICY APPENDIX I – DATASET DETAILS (to be completed by the data producer before passing to applicant) Dataset reference (EGA Study ID and Dataset Details) EGAS00001005593: Single-cell proteo-genomic reference maps of the human hematopoietic system Name of project that created the dataset Single-cell proteo-genomic reference maps of human blood and bone marrow that quantitatively link the expression of up to 197 surface markers to cellular identities and biological processes across all major hematopoietic cell types in healthy aging and leukemia. Contact details of Data access committee members Name: Simon Haas Institution: MDC/Charité/DKFZ Affiliation Title/Position: Group leader and Senior Scientist Email: Simon.Haas@mdc-berlin.de Business address: Hannoversche Straße 28; 10115 Berlin; Germany Telephone: +49 30 9406 1428 Lars Velten CRG Group leader and Senior Scientist lars.velten@crg.eu Carrer del Dr. Aiguader, 88, 08003 Barcelona, Spanien +34 93 316 0185 Andreas Trumpp DKFZ Division head a.trumpp@dkfz.de Im Neuenheimer Feld 280 69120 Heidelberg Germany +49 6221 42 3901 Tanja Heimberger DKFZ Scientific Coordinator tanja.heimberger@dkfz-heidelberg.de Im Neuenheimer Feld 280 69120 Heidelberg Germany +49 6221 42 3442 Contact details of Data producer(s) Name Institution/ Affiliation Title/ Position Email Business address Telephone Simon Haas MDC/Charité/DKFZ Group leader and Senior Scientist Simon.Haas@mdc-berlin.de Hannoversche Straße 28 10115 Berlin Germany +49 30 9406 1428 Specific limitations on areas of research Research with the goal of identifying characteristics of the patient not related to the leukemia (such as surname inference and ancestry research) are excluded. The use of the data for projects not related to cancer research is excluded, exceptions may apply in the context of research aiming to develop new bioinformatics methods. Minimum protection measures required Unlimited sharing for the purpose of scientific research upon reasonably justified requests, provided the data will not be made publicly available. A documented analysis of risks involved with processing of data shared hereunder, including development and implementation of suitable mitigation measures ensuring an appropriate level of security, including confidentiality, as well as compliance with applicable legal frameworks. File access: Data can be held in unencrypted files on an institutional compute system, with Unix user group read/write access for one or more appropriate groups but not Unix world read/write access behind a secure firewall. Laptops holding these data should have password protected logins and screenlocks (set to lock after 5 min of inactivity). If held on USB keys or other portable hard drives, the data must be encrypted. APPENDIX II – PROJECT DETAILS (to be completed by the Requestor) Details of dataset requested i.e., EGA Study and Dataset Accession Number Brief abstract of the Project in which the Data will be used (500 words max) All Individuals who the User Institution to be named as registered users Name of Registered User Email Job Title Supervisor* All Individuals that should have an account created at the EGA Name of Registered User Email Job Title APPENDIX III – PUBLICATION POLICY Data producer intend to publish the results of their analysis of this dataset and do not consider its deposition into public databases to be the equivalent of such publications. Data producer anticipate that the dataset could be useful to other qualified researchers for a variety of purposes. However, some areas of work are subject to a publication moratorium. The publication moratorium covers any publications (including oral communications) that describe the use of the dataset. For research papers, submission for publication should not occur until 36 months after these data were first made available on the relevant hosting database, unless Data producer has provided written consent to earlier submission. In any publications based on these data, please describe how the data can be accessed, including the name of the hosting database (e.g., The European Genome-phenome Archive at the European Bioinformatics Institute) and its accession numbers (e.g., EGAS00000000029), and acknowledge its use in a form agreed by the User Institution with Data producer.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001005593 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00005475303 fastq.gz 3.4 GB
EGAF00005475304 fastq.gz 5.0 GB
EGAF00005475305 fastq.gz 1.3 GB
EGAF00005475306 fastq.gz 1.7 GB
EGAF00005475307 fastq.gz 3.5 GB
EGAF00005475308 fastq.gz 5.5 GB
EGAF00005475309 fastq.gz 1.7 GB
EGAF00005475310 fastq.gz 2.2 GB
EGAF00005475311 fastq.gz 3.6 GB
EGAF00005475312 fastq.gz 5.6 GB
EGAF00005475313 fastq.gz 9.6 GB
EGAF00005475314 fastq.gz 15.5 GB
EGAF00005475315 fastq.gz 3.5 GB
EGAF00005475316 fastq.gz 5.5 GB
EGAF00005475317 fastq.gz 3.4 GB
EGAF00005475318 fastq.gz 5.0 GB
EGAF00005475319 fastq.gz 2.4 GB
EGAF00005475320 fastq.gz 3.6 GB
EGAF00005475321 fastq.gz 3.7 GB
EGAF00005475322 fastq.gz 5.4 GB
EGAF00005475323 fastq.gz 3.2 GB
EGAF00005475324 fastq.gz 5.4 GB
EGAF00005475325 fastq.gz 2.5 GB
EGAF00005475326 fastq.gz 3.7 GB
EGAF00005475327 fastq.gz 3.5 GB
EGAF00005475328 fastq.gz 5.0 GB
EGAF00005475329 fastq.gz 3.6 GB
EGAF00005475330 fastq.gz 5.6 GB
EGAF00005475331 fastq.gz 3.8 GB
EGAF00005475332 fastq.gz 5.8 GB
EGAF00005475333 fastq.gz 2.4 GB
EGAF00005475334 fastq.gz 3.6 GB
EGAF00005475335 fastq.gz 3.6 GB
EGAF00005475336 fastq.gz 4.2 GB
EGAF00005475337 fastq.gz 9.6 GB
EGAF00005475338 fastq.gz 15.5 GB
EGAF00005475339 fastq.gz 3.4 GB
EGAF00005475340 fastq.gz 5.1 GB
EGAF00005475341 fastq.gz 3.4 GB
EGAF00005475342 fastq.gz 5.0 GB
EGAF00005475343 fastq.gz 5.5 GB
EGAF00005475344 fastq.gz 8.2 GB
EGAF00005475345 fastq.gz 9.0 GB
EGAF00005475346 fastq.gz 13.6 GB
EGAF00005475347 fastq.gz 18.9 GB
EGAF00005475348 fastq.gz 21.8 GB
EGAF00005475349 fastq.gz 3.4 GB
EGAF00005475350 fastq.gz 5.0 GB
EGAF00005475351 fastq.gz 11.1 GB
EGAF00005475352 fastq.gz 15.8 GB
EGAF00005475353 fastq.gz 3.5 GB
EGAF00005475354 fastq.gz 5.6 GB
EGAF00005475355 fastq.gz 7.0 GB
EGAF00005475356 fastq.gz 10.7 GB
EGAF00005475357 fastq.gz 2.6 GB
EGAF00005475358 fastq.gz 3.9 GB
EGAF00005475359 fastq.gz 5.0 GB
EGAF00005475360 fastq.gz 7.8 GB
EGAF00005475361 fastq.gz 5.4 GB
EGAF00005475362 fastq.gz 9.3 GB
EGAF00005475363 fastq.gz 2.5 GB
EGAF00005475364 fastq.gz 3.7 GB
EGAF00005475365 fastq.gz 2.5 GB
EGAF00005475366 fastq.gz 3.7 GB
64 Files (374.7 GB)