Platelets sequester extracellular DNA, capturing tumour-derived and free fetal DNA

Type: Whole Genome Sequencing
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

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Dataset ID	Description	Technology	Samples
EGAD00001009856	This dataset consists of WGS from six patients with a recent diagnosis of gastrointestinal adenocarcinoma. DNA-Seq libraries were prepared from platelet DNA (pDNA) and plasma cfDNA obtained from the same peripheral blood sample. pDNA was size selected into 2 groups (small, s-pDNA: less than 600 bp and long, b-pDNA: greater than 600 bp). The short fragments were further cleaned to remove <100 bp fragments and large platelet fragments were fragmented via sonication. Libraries were then prepared using the NEBNext Ultra II DNA Sample Preparation Kit for Illumina according to the manufacturer’s protocol and sequenced on an Illumina NextSeq 500 (300 cycle PE) at low-pass (0.1X) for all samples, and 10X for the cfDNA and s-pDNA, using four lanes in each sample.	NextSeq 500	20

Publications	Citations
Platelets sequester extracellular DNA, capturing tumor-derived and free fetal DNA. Murphy L, Inchauspé J, Valenzano G, Holland P, Sousos N, Belnoue-Davis HL, Li R, Jooss NJ, Benlabiod C, Murphy E, Etzioni Z, Shepherd E, Denly L, Biswas S, Chen L, O'Sullivan J, Rimmer MP, Khan AO, Karali CS, Nasreddin N, Hitchcock IS, Koupenova M, Kriaucionis S, Hughes JR, O'Neill E, Vatish M, Rees P, Leedham S, Desborough M, Mead AJ, Schuster-Böckler B, Gregory CD, Psaila B. Science 389: 2025 eadp3971	5

Publications

Citations

Platelets sequester extracellular DNA, capturing tumor-derived and free fetal DNA.
Murphy L, Inchauspé J, Valenzano G, Holland P, Sousos N, Belnoue-Davis HL, Li R, Jooss NJ, Benlabiod C, Murphy E, Etzioni Z, Shepherd E, Denly L, Biswas S, Chen L, O'Sullivan J, Rimmer MP, Khan AO, Karali CS, Nasreddin N, Hitchcock IS, Koupenova M, Kriaucionis S, Hughes JR, O'Neill E, Vatish M, Rees P, Leedham S, Desborough M, Mead AJ, Schuster-Böckler B, Gregory CD, Psaila B. Science 389: 2025 eadp3971