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Cold Ischemia Study

This study aimed to evaluate the impact of cold ischemia time on the expression of cancer-related genes, particularly those involved in hypoxia, apoptosis, and epithelial-to-mesenchymal transition (EMT). We performed RNA sequencing on 54 normal colon mucosa samples from nine patients undergoing colorectal cancer surgeries, freezing samples at predefined intervals ranging from 0 to 60 minutes. A total of 44 differentially expressed genes (DEGs) (p < 0.05) were identified when comparing samples frozen immediately (T0) with those frozen after 60 minutes (T5).

Type: Other
Archiver: European Genome-phenome Archive (EGA)

2 Datasets 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD00001015661	A total of 54 samples derived from 9 patients and frozem in 6 timepoints (T0 - immediately after resection, T1 - 10 minutes after resection, T2 - 20 minutes after resection, T3 - 30 minutes after resection, T4 - 45 minutes after resection, T5 - 60 minutes after resection) to assess the impact of ischemia on gene expression.	HiSeq X Ten	54
EGAD00001015809	Dataset for H3K27me3 ChIP-seq data of 31 neuroblastoma tumor samples. The sequencing was performed on Illumina HiSeq 2000 and Illumina HiSeq 2500	Illumina HiSeq 2000 Illumina HiSeq 2500	30

Publications	Citations
The effect of cold ischemia time on hypoxia, EMT, and apoptosis pathways in normal colon mucosa. Duzowska K, Opiełka M, Drężek-Chyła K, Kostecka A, Horbacz M, Skokowski J, Rostkowska O, Kobiela J, Kalinowski L, Dumanski JP, Piotrowski A, Jąkalski M, Filipowicz N. PLoS One 21: 2026 e0321432	0