Human scMultiome data (GEX and ATAC paired) from CD34+ Bone Marrow (BM) and mobilized peripheral blood (mPB), including CITE-Seq data from mPB CD34+ samples

We analyzed healthy CD34+ samples from Bone Marrow (BM) and mobilized Peripheral Blood (mPB) in gene therapy experiments within the X-PAND project. BM samples underwent scMultiome analysis, providing paired GEX and ATAC data. For the mPB samples, both scMultiome and CITE-Seq were performed on the same donors, offering additional protein marker information. These datasets allow for an in-depth exploration of chromatin accessibility, gene expression, and surface protein profiling in hematopoietic progenitor cells from healthy donors.

Type: Gene Regulation Study
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000001108	This dataset contains five bone marrow samples and three mobilised peripheral blood ones from different healthy individuals. The three bone marrow samples have been sequence with the 10x multiome kit using Illumina sequencers. The three mobilised peripheral blood ones have been split in two aliquots, one for 10x multiome and the other for 10x CITE-seq. Both libraries types have been sequenced with an Illumina sequencer. Raw data has been processed with cell-ranger and map to the human genome (GRCh38) to obtain the bam files.	Illumina NovaSeq 6000	18

Publications	Citations
Uncovering the regulatory landscape of early human B cell lymphopoiesis and its implications in the pathogenesis of B-ALL. Planell N, Martínez-de-Morentin X, Mouzo D, Lara-Astiaso D, Vilas-Zornoza A, Martín-Uriz PS, Alignani D, Paiva B, Maillo A, Kurowska A, Berastegui N, Garcia-Olloqui P, Urdangarin A, Noori P, Ortega-Legarreta A, Hernaez M, Lagani V, Kiani N, Merkenschlager M, Ezponda T, Martín-Subero JI, Ramírez RN, Tegner J, Prosper F, Gomez-Cabrero D. Sci Adv 11: 2025 eadw3110	0

Publications

Citations

Uncovering the regulatory landscape of early human B cell lymphopoiesis and its implications in the pathogenesis of B-ALL.
Planell N, Martínez-de-Morentin X, Mouzo D, Lara-Astiaso D, Vilas-Zornoza A, Martín-Uriz PS, Alignani D, Paiva B, Maillo A, Kurowska A, Berastegui N, Garcia-Olloqui P, Urdangarin A, Noori P, Ortega-Legarreta A, Hernaez M, Lagani V, Kiani N, Merkenschlager M, Ezponda T, Martín-Subero JI, Ramírez RN, Tegner J, Prosper F, Gomez-Cabrero D. Sci Adv 11: 2025 eadw3110