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In vitro depletion of Caveolin-1 in human plasmablast cells

In humans, unlike in mice, Cav-1 is expressed not only after B-cell activation but also in PC progenitors, plasmablasts, and mature PCs. To assess its functional role during B-cell differentiation, we used our previously published in vitro B-cell differentiation model and transfected day-5 cells with CAV1-targeting siRNAs. This resulted in a significant reduction of both CAV1 mRNA and protein levels by day 7, compared with scrambled siRNA controls. Bulk RNA-seq was then performed on day-7 plasmablasts transfected with siRNA-CAV1 versus siRNA control to compare their transcriptomic profiles (n = 4 experiments).

Type: RNASeq
Archive: European Genome-phenome Archive (EGA)

1 Dataset

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Dataset ID	Description	Technology	Samples
EGAD50000002119	On day 7, B cells previously electroporated on day 5 with siControl or siCAV1 (n = 4 donors) were processed for bulk RNA-seq. RNA integrity was verified using a Bioanalyzer 2100 (Agilent). Libraries were prepared and sequenced at Institut Curie (Paris) on an Illumina NovaSeq 6000 (2 × 100 bp; ≥50 million reads/sample; ≥80% ≥Q30). Reads were quality-checked using FastQC v0.11.5	Illumina NovaSeq 6000	8