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Establishment of germline-encoded antibodies during primary dengue infection

This study supports the manuscript “Establishment of germline-encoded antibodies during primary dengue infection” and characterizes longitudinal B cell receptor repertoire dynamics during controlled primary DENV1 infection. Bulk BCR sequencing was performed on whole-blood total RNA from flavivirus-naïve participants in a DENV1 live-virus human challenge study. Samples were collected at days 0, 8, 10, 14, and 28 post-infection, corresponding to pre-infection, early viremic, peak viremic/acute, late viremic/critical-phase, and convalescent timepoints. Libraries were prepared from 1 µg RNA per sample using the NEBNext Immune Sequencing Kit with BCR/IGH-specific amplification and sequenced on an Element Biosciences AVITI system using 300PE chemistry.

Type: Transcriptome Sequencing
Archive: European Genome-phenome Archive (EGA)

1 Dataset

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Dataset ID	Description	Technology	Samples
EGAD50000002823	This dataset contains bulk BCR/IGH adaptive immune receptor repertoire sequencing data from longitudinal whole-blood RNA samples collected during a controlled primary DENV1 human challenge study. Raw paired-end FASTQ files are provided for available participant-timepoint samples, with associated processed clone-pass repertoire tables generated using the nf-core/airrflow/Immcantation workflow. The dataset includes samples from participants 201-209 across available days 0, 8, 10, 14, and 28, excluding missing participant-timepoints. The data support analysis of BCR clonal expansion, isotype usage, somatic hypermutation, and convergent antibody responses after dengue infection.	Element AVITI	43