Tampere University Hospital Leukemia Reserach Data Access Committee

Dac ID Contact Person Email Access Information
EGAC00001001551 Olli Lohi olli [dot] lohi [at] tuni [dot] fi No additional information is available

This DAC controls 2 datasets:

Dataset ID Description Technology Samples
EGAD00001006074 Leukemic bone marrow in primary ETV6-RUNX1 positive acute lymphoblastic leukemia samples (Six diagnostic and two 15 days after treatment) using Chromium 3' single cell RNA-seq. Samples sequenced on 1-3 lanes and raw fastq files provided for each sample. Illumina HiSeq 3000 8
EGAD00001006406 RNA was extracted from eight diagnostic ETV6-RUNX1 positive acute lymphoblastic leukemia samples collected in PAXgene blood RNA tubes using PAXgene Blood RNA kit (cat #762174, Qiagen GmbH, Hilden, Germany), following the version 2 instructions for manual purification. Samples were processed with Globin-Zero Gold rRNA Removal Kit (Illumina) and directional libraries were prepared using NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs). The library preparation and paired end (150 bp) sequencing were performed by Novogene (HK) Company Limited (Hong Kong, China) using Illumina Novaseq 6000 aiming at 70 million read pairs per sample. Illumina NovaSeq 6000 8