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Assessment of epigenetic variation in human iPS cells-Medip

n order to progress human induced pluripotent stem cells (hiPSCs) towards the clinic, several outstanding questions must be addressed. It is possible to reprogram different somatic cell types into hiPSCs and from studies in the mouse, it appears that an epigenetic memory of the starting cell type is carried over to hiPSCs. However a comprehensive comparative study of the characteristics of these hiPSCs has been missing from the literature. Importantly studies which aimed to address these aspects of hiPSCs have used cells from different patients. In order to avoid this important confounding variable and to keep the genetic background constant, tissue samples were procured from the patients and reprogrammed to iPS cells. The methylation status of these iPS cells will be compared. Protocol: Primary cell cultures were generated and reprogrammed to iPS cells. DNA was extracted and immunoprecipitated using anti-methyl cytosine and anti-hydroxymethyl cytosine antibodies. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001000741 Epigenetics

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00000519111 cram 160.0 MB
EGAF00000519112 cram 86.4 MB
EGAF00000519113 cram 155.0 MB
EGAF00000519114 cram 355.1 MB
EGAF00000519115 bam 86.6 kB
EGAF00000519116 bam 105.5 kB
EGAF00000519117 bam 326.5 kB
EGAF00000519118 bam 418.3 kB
EGAF00000519119 bam 475.4 MB
EGAF00000519120 bam 432.0 MB
EGAF00000519121 bam 398.1 MB
EGAF00000519122 bam 403.4 MB
12 Files (2.5 GB)