Dataset

Epigenome maps of time-resolved monocyte to macrophage differentiation and innate immune memory

Dataset ID Technology Samples
EGAD00001002693 Illumina HiSeq 2000,NextSeq 500,unspecified 71

Dataset Description

Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components such as LPS. We apply an integrated epigenomic approach to characterize the molecular events involved in LPS-induced tolerance in a time dependent manner. ChIP-seq, RNA-seq, WGBS and ATAC-seq data were generated. This analysis identified epigenetic programs in tolerance and trained macrophages, and the potential transcription factors involved.

Experimental set-up
Time-course in vitro culture of human monocytes. Two innate immune memory states can be induced in culture through an initial exposure of primary human monocytes to either LPS or BG for 24 hours, followed by removal of stimulus and differentiation to macrophages for an additional 5 days. Cells were collected at baseline (day 0), 1 hour, 4 hour, 24 hour and 6 days.

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

Blueprint Data Access Committee
Contact person: Paul Flicek
Email: blueprint-dac [at] ebi [dot] ac [dot] uk
More details: EGAC00001000135

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