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LLDeep DAG2+: scRNA-seq in PBMCs

Single cell transcriptomics of PBMCs of 47 donors from the Lifelines Deep cohort (general population, Northern part of the Netherlands). Cells of five or six different donors were pooled together in one sample pool, resulting in eight different sample pools. In total, 28.855 cells were captured and their transcriptomes were sequenced to an average depth of 74k. Genotype data was available for each donor, which allowed us to use the Demuxlet method that uses variable SNPs between the pooled individuals to determine which cell belongs to which individual. Since genotype information is lacking of 2 individuals, the transcriptome of only 45 individuals could be retrieved.

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The Lifelines DEEP DAG2+ Project (“LLDEEP DAG2+”): data access policy

The Lifelines DEEP DAG2+ Project (“LLDEEP DAG2+”): Access to Data General principles Funded by an ERC starting grant ‘Defining how environmental factors influence the downstream effects of immune-mediated disease risk-SNPs’ the department of Genetics in the University Medical Center Groningen has sequenced single peripheral blood mononuclear cells (PBMCs) of 47 participants of the Dutch Lifelines cohort in the LLDEEP DAG2+ project (hereinafter referred to as “Project” or “LLDEEP DAG2+”). The Project is led by a research group (hereinafter also referred to as the Research Group) from the University Medical Center Groningen (“UMCG”). The primary goal of the LLDEEP DAG2+ project is to get insight in the relations between genetic variation and transcriptional response of individual cells. The Research Group is willing to consider applications from third party researchers for access to the anonymized sequence data generated by the Project. Access to Project Data will be granted to qualified researchers for approved use and will be governed by the provisions laid out in the informed consent of the LLDEEP DAG2+ cohort, the Data Access Procedure and the terms of the Data Access Agreement. A ‘qualified researcher’ refers to a senior investigator who is employed or legitimately affiliated with an academic, non-profit or government institution and who has a track record in the field. Access to Project Data is available by application to the LLDEEP DAG2+ Data Access Committee. Researchers granted access to project data must feedback the results of their research to LLDEEP DAG2+, after publication in accordance with the LLDEEP DAG2+ publication policy set forth in the Data Access Agreement. Access is conditional upon availability of data and on signed agreement by the researcher(s) and the responsible employing institution to abide by the policies and conditions related to publication, data ownership, data return, intellectual property rights, data disposal, ethical approval, confidentiality and commercialization referred herein. Description of the available data This Project includes single cell RNA-sequencing (scRNA-seq) data from ~30,000 PBMCs from 47 Lifelines DEEP participants (25 males and 22 females) from the general population of the northern part of the Netherlands (Tigchelaar, E. F. et al. Cohort profile: LifeLines DEEP, a prospective, general population cohort study in the northern Netherlands: study design and baseline characteristics. BMJ Open 5, e006772-2014-006772 (2015)). The cohort comprised predominantly Dutch participants; 93.7% had both parents born in the Netherlands. The available dataset contains scRNA-seq sequencing data. On average ~550 PBMCs of each individual were analyzed using scRNA-sequencing on a HiSeq4000, resulting in about 511 Gb of data (239 Gb BAM-files and 272 Gb fastq-files). An additional text file is included in which the Demuxlet method was used to couple each cell barcode to an individual/sample-id (using genotype data). Access to data is available by application to the LLDEEP DAG2+ Data Access Committee. Access to data will be granted to qualified investigators for appropriate use. Data Access Committee Applications for access to Project Data must be made to the LLDEEP DAG2+ Data Access Committee (DAC). The LLDEEP DAG2+ DAC comprises representatives of the following members: Prof. Dr. L. Franke, Dr. M.G.P. van der Wijst, J.A.M. Dekens, D.H. de Vries. Contact info address: j.a.m.dekens@umcg.nl. Data Access Procedure 1. Application for Access The handling of applications, the issue of Project Data and any associated operations, administration and audits will be performed by the UMCG on behalf of the Research Group. Applications for Access to Project Data must be submitted using the Application Form for Access to LLDEEP DAG2+ Data. The information disclosed in the application will be treated as confidential and will only be disclosed to the persons evaluating the application. All incoming applications will be documented, including any conjunct agreements with the applicants. Unless explicitly consented for by the applicant, this information will not be used for purposes other than evaluation of the application. In cases where an application contains classified plans, know-how or trade secrets, the applicant may request the conclusion of a separate confidentiality agreement. 2. Multiple applications Applicants agree to use the Project Data for the approved purpose and project described in the application; use of the data for a new purpose or project will require a new application and approval. The DAC will consider applications that include named collaborators, but each Institution must sign a separate Data Access Agreement. In the event an applicant wishes to share the Project Data with additional collaborators not previously approved, these additional collaborators must make a separate application for access to the Project Data. In the event two or more applications overlap, the DAC may propose the respective applicants to align their applications. 3. Assessment criteria Applications which seek to reserve Project Data for unspecified research goals will not be taken into consideration. Applications for access to Project Data must be Specific, Measurable, Attainable, Resourced and Timely (SMART). Specifically, the LLDEEP DAG2+ DAC will assess each application to determine whether: i. it has been submitted by a qualified researcher or researchers, who is/are employed by or legitimately affiliated with a recognized research institution that can provide institutional responsibility for appropriate research governance; ii. its proposed use of the Project Data is in accordance with and meets the objectives of the LLDEEP DAG2+ Project and the objectives of the associated cohorts and collections; iii. its proposed use of the Project Data constitutes research in the context of the individual sample donor consent process, and is likely to be understood as such by the individual sample donors; iv. it would breach any of the ethical permissions or restrictions in the consent form; v. all required ethico-legal approvals, restrictions and commitments for the proposed use of the Project Data have been obtained and adhered to; vi. the proposed research has any adverse potential impact, specifically whether the proposed research could affect minorities; vii. the nature of the funding of the application and the applicant; viii. the research proposal has been peer-reviewed and if not, whether the proposal satisfies applicable scientific standards; ix. the requested Project Data are, quantitatively and qualitatively, suitable and not excessive for the applicant’s proposed research; x. there are any similar applications pending or granted; xi. the proposed use of the Project Data has the potential to produce information that will enable identification of the individual sample donors. xii. the applicant has warranted that he has obtained all required ethico-legal approvals, restrictions and commitments, without limitation his/her data subject consent and approval of his/her pertinent medical ethical review board, for his/her proposed use of his/her data in combination with the Project Data. 4. Access decision The DAC will decide on an application within six weeks after it has received all pertinent information. If the DAC grants an application, additional agreements may be made regarding authorships along the lines set forth in the Publication Policy that will be send upon application. Rejections of applications will be motivated. Mode of Access to Data; restrictions and authentication The Research Group will make the anonymized Project Data available by web based provisions of EGA. Issue of Project Data may be subject to restrictions pursuant to data subject’s or ethics committee’s consent. Prior to issue of Project Data applicants will be subject to authentication. The issue of Project Data and the Data Access Agreement will be administered by UMCG. Data Access Conditions Access to Project Data is conditional on prior receipt by the Research Group of the Data Access Agreement sent upon receipt of the application, signed and dated by the applicant(s) and the responsible employing Institution(s).   Data Access Agreement This agreement governs the terms on which access will be granted to the Project Data generated by the LLDEEP DAG2+ Project. In signing this agreement, You are agreeing to be bound by the terms and conditions of access set out in this agreement. For the sake of clarity, the terms of access set out in this agreement apply both to the User and the User’s Institution (as defined below). User Institution and User are referred to within the agreement as “You” and “Your” and shall be construed accordingly. Definitions: “Data Subject” refers to the person whose sample has been used to generate Project Data. “Project” refers to the LLDEEP DAG2+ project as described in the ERC starting grant ‘Defining how environmental factors influence the downstream effects of immune-mediated disease risk-SNPs’ (http://cordis.europa.eu/project/rcn/196768_en.html). “Project Data” refers to the single cell RNA-sequencing data generated in the LLDEEP DAG2+ cohort. “Publications” refers to, without limitation, articles published in print journals, electronic journals, reviews, books, posters and other written and verbal presentations of research. “Research Group” refers to the LLDEEP DAG2+ Research Group from the University Medical Center Groningen (UMCG). “User” refers to a researcher who has successfully applied for access to Project Data. “User Institution” refers to the organization with which the User is employed, affiliated or enrolled. Terms and Conditions: In signing this agreement: 1. You agree to use Project Data only for the advancement of scientific and medical research. 2. You agree not to use, and not to allow use of, Project Data or any part thereof for the creation of products or services for sale or for any commercial purpose, whether sale by You or by third parties. 3. You acknowledge that the Project Data You receive is anonymized and that the Research Group will under no circumstances provide You with any means to identify any Data Subject. You agree not to use Project Data to identify or contact individual Data Subjects. You agree to preserve, at all times, the confidentiality of information and Project Data. In particular, You undertake not to use, or attempt to use Project Data to compromise or otherwise infringe the confidentiality of information on Data Subjects and their right to privacy. 4. You agree to disclose Project Data only to those of Your employees who strictly need access to it for the purpose of your approved study (Authorized Recipients), and who are bound by a duty of confidentiality which is at least as strict as provided for in this agreement. 5. You agree to ensure that any Authorized Recipient who has access to Project Data is advised of the confidentiality of Project Data. Upon request by the Research Group, you agree to furnish the Research Group the names of all Authorized Recipients who have gained access to Project Data. You agree that You are responsible for all breaches of confidentiality and of the terms of this agreement by your Authorized Recipients. 6. You agree to store Project Data on a computer with adequate security controls that prevent unauthorized access to or loss of Project Data and to maintain appropriate control over the Project Data. You represent and warrant that You have in place, and You agree that You will keep having in place, state of the art technical and organizational security measures preventing unauthorized access and loss of Project Data or other forms of unlawful processing of Project Data, including without limitation, physical security measures, access controls, security and privacy technologies, security checks in relation to personnel, security incident response management and audit arrangements. You agree to notify forthwith the administrator of the Project, UMCG, of any violations of the foregoing. 7. You represent and warrant that all required ethico-legal approvals, restrictions and commitments, without limitation data subject consent and approval of the pertinent medical ethical review board, for Your proposed use of Your data in combination with the Project Data, have been obtained and adhered to. 8. In the event the Project Data is issued to You subject to any Data Subject’s restrictions, you hereby assume the obligation to abide by these restrictions as Your own obligation towards the Data Subject. You agree that Data Subjects can enforce such restrictions as well as any pertinent provisions of this agreement as third-party beneficiaries and, in the event they choose to do so, that You will not object to Data Subjects being represented by an association or other bodies if they so wish. You agree that a Data Subject who has suffered damage(s) as a result of any violation by You of pertinent provisions in this agreement or of applicable legislation, is entitled to receive compensation from You for the damage(s) suffered. You agree that, in the event of such a violation, the Data Subject may bring an action before a court within the Data Subject’s jurisdiction against You. 9. You agree not to attempt to link Project Data to other information or archive data available for the data sets provided to You under this agreement, even if access to that data has been formally granted to you, or it is freely available without restriction, without the required consents and approvals. You agree to retain control over Project Data, and NOT TO DISTRIBUTE (individual-level or aggregated) Project Data provided under this agreement, in any form, to any entity or individual. 10. You agree not to transfer, share or disclose Project Data, in whole or in part, to or with anybody else, whether or not he or she is affiliated with Your institution, unless he or she is a named and approved collaborator under the LLDEEP DAG2+ Data Access Procedure. 11. You agree to use Project Data for the purpose and project described in your application (nr. ………………………) approved by the LLDEEP DAG2+ Data Access Committee; use of the data for a new purpose or project or by another researcher will require a new application and approval. Substantive modification of the original application requires submission of an amendment to the original application. 12. You agree to, when analyses result in newly estimated variables at an individual level, submit these new variables for archiving with the Research Group. This should be done no later than that the related paper will be submitted for publication. 13. You accept that Project Data will be reissued from time to time, with suitable versioning. If the reissue is at the request of sample donors and/or subject to other ethical scrutiny, You will destroy earlier versions of the Project Data. 14. You agree to destroy the Project Data after the resulting paper has been published. Upon destruction of data you will send a written confirmation. You agree to abide by the terms outlined in the Research Group 'Publication Policy' as set out in Schedule 1. 15. You agree to acknowledge in any work based in whole or in part on Project Data, the published paper from which the Project Data derives, the version of the data, and the role of the Research Group and the relevant primary collectors and their funders, as per the wording provided in the Publication Policy set forth in Schedule 1. 16. You accept that the Research Group, the original Project Data generators and the funders of the Project Data: a) bear no legal responsibility whatsoever for the accuracy, completeness or comprehensiveness of the Project Data; and b) accept no liability for direct, indirect, consequential or incidental, damages or losses arising from any use of Project Data by anyone, including without limitation any clinical use and any commercial use thereof, or arising from the unavailability of, or break in access to, the Project Data for whatever reason. 17. You understand and acknowledge that Project Data are protected by copyright, database right and other intellectual property and proprietary rights, and that duplication, sale, licensing or any other form of disposing or distribution or making available, of all or part of the Project Data on any media is not permitted. 18. You acknowledge that the Project Data are made available to you under this agreement on the title of un- assignable, non-exclusive “loan” (“leen”). You recognize that nothing in this agreement shall operate to transfer to You any rights, including but not limited to intellectual property rights and database rights, in or relating to Project Data. 19. You accept that this agreement will terminate immediately upon any breach of this agreement by You and You will be required to destroy any Project Data held. 20. You accept that it may be necessary for the Research Group or its appointed agent to alter the terms of this agreement from time to time in order to address new concerns. In this event, the Research Group or its appointed agent will contact You to inform You of any changes and You agree that Your continued use of Project Data shall be dependent on the parties entering into a new version of the agreement. 21. You agree that You will submit a report to the LLDEEP DAG2+ Data Access Committee, if requested, on completion of your study of the Project Data. Such report will include the data resulting from your study of the Project Data. The LLDEEP DAG2+ Data Access Committee agrees to treat the report and all information, data, results, and conclusions contained within such report as confidential information. 22. You accept that Project Data may be protected by and subject to national and international laws and that You are responsible for ensuring compliance with any such applicable law. The LLDEEP DAG2+ Data Access Committee reserves the right to request and inspect Your data security and management documentation to ensure the adequacy of data protection measures in countries that have no national laws comparable to that which pertain in the EEA (European Economic Area). 23. This agreement shall be construed, interpreted and governed by the laws of the Netherlands and shall be subject to the exclusive jurisdiction of the district court of Northern Netherlands. 24. This agreement may be executed in two or more counterparts, each of which shall be considered an original instrument. Each counterpart will be considered a valid and binding original, and all counterparts taken together shall constitute one and the same agreement. Faxed copies and scanned copies of original signatures shall be deemed as effective as original signatures. Once signed, any reproduction of this agreement made by reliable means (e.g., photocopy, facsimile, scan) is considered an original. Signed by: ……………………………………………………………. ………………………………….……………………………………. Name of Applicant: Legal representative of Applicant’s Institution: Affiliation of Applicant with Institution: Name of Applicant’s Institution: ……………………………………………………………. ……………………………………………………………………….. Place:………………………………………………….. Place:……………………………………………………………… Date:…………………………………………………… Date:……………………………..……………………………….. Stamp or seal of Applicant’s Institution: ………………………………………………………………………..  SCHEDULE 1 Publication Policy Publications involving Project Data that do not fall under “Use case 3” as specified below, should be submitted to the LLDEEP DAG2+ Research Group for review, including review of proper authorship allocation and proper acknowledgments, before submission. The LLDEEP DAG2+ Research Group will differentiate between the following three use cases: Use case 1. Primary papers by the Project (raw LLDEEP DAG2+ data is used). Use case 2. Papers which include analyses by the Research Group using summary statistics of LLDEEP DAG2+ Data. Specific individuals of the Project, who have contributed substantially to the analyses or the paper, may be listed explicitly as well (to be agreed upon by the Applicant and the Research Group). Use case 3. Papers which include analyses of LLDEEP DAG2+ Data, made available through provisions of EGA. In general, no co-authorship will be needed, although it is emphasized that the open-access Data is made available on an "as is" basis and subject to the condition that any use thereof shall not in any way inhibit or restrict the free use thereof by any third party. For each of the above use cases, authors who use Project Data must: 1. acknowledge the LLDEEP DAG2+ Research Group and ERC Starting grant using the following wording: “We thank the participants and the staff of Lifelines-DEEP DAG2+ for their collaboration. Funding for the project was provided by the ERC Starting Grant #637640 awarded to Prof. Dr. L. Franke.” 2. acknowledge, by reference, the funders of the sample collections from which the Project Data have been derived: a. The Lifelines Cohort Study (http://www.lifelines.nl 3. cite the relevant primary LLDEEP DAG2+ publication

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001002560 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00001706872 bam 34.6 GB
EGAF00001706873 bam 32.6 GB
EGAF00001706874 bam 33.8 GB
EGAF00001706875 bam 29.8 GB
EGAF00001706876 bam 33.2 GB
EGAF00001706877 bam 28.7 GB
EGAF00001706878 bam 29.5 GB
EGAF00001706879 bam 30.9 GB
EGAF00001706880 fastq.gz 783.5 MB
EGAF00001706881 fastq.gz 3.7 GB
EGAF00001706882 fastq.gz 794.5 MB
EGAF00001706883 fastq.gz 3.8 GB
EGAF00001706884 fastq.gz 801.7 MB
EGAF00001706885 fastq.gz 3.9 GB
EGAF00001706886 fastq.gz 786.0 MB
EGAF00001706887 fastq.gz 3.8 GB
EGAF00001706888 fastq.gz 795.9 MB
EGAF00001706889 fastq.gz 3.9 GB
EGAF00001706890 fastq.gz 768.4 MB
EGAF00001706891 fastq.gz 3.7 GB
EGAF00001706892 fastq.gz 779.3 MB
EGAF00001706893 fastq.gz 3.8 GB
EGAF00001706894 fastq.gz 803.9 MB
EGAF00001706895 fastq.gz 4.0 GB
EGAF00001706896 fastq.gz 753.9 MB
EGAF00001706897 fastq.gz 3.5 GB
EGAF00001706898 fastq.gz 764.6 MB
EGAF00001706899 fastq.gz 3.6 GB
EGAF00001706900 fastq.gz 768.9 MB
EGAF00001706901 fastq.gz 3.7 GB
EGAF00001706902 fastq.gz 754.5 MB
EGAF00001706903 fastq.gz 3.6 GB
EGAF00001706904 fastq.gz 760.1 MB
EGAF00001706905 fastq.gz 3.7 GB
EGAF00001706906 fastq.gz 735.6 MB
EGAF00001706907 fastq.gz 3.6 GB
EGAF00001706908 fastq.gz 741.9 MB
EGAF00001706909 fastq.gz 3.6 GB
EGAF00001706910 fastq.gz 767.6 MB
EGAF00001706911 fastq.gz 3.8 GB
EGAF00001706912 fastq.gz 766.4 MB
EGAF00001706913 fastq.gz 3.6 GB
EGAF00001706914 fastq.gz 776.0 MB
EGAF00001706915 fastq.gz 3.7 GB
EGAF00001706916 fastq.gz 782.6 MB
EGAF00001706917 fastq.gz 3.8 GB
EGAF00001706918 fastq.gz 768.2 MB
EGAF00001706919 fastq.gz 3.7 GB
EGAF00001706920 fastq.gz 776.7 MB
EGAF00001706921 fastq.gz 3.8 GB
EGAF00001706922 fastq.gz 751.0 MB
EGAF00001706923 fastq.gz 3.6 GB
EGAF00001706924 fastq.gz 760.2 MB
EGAF00001706925 fastq.gz 3.7 GB
EGAF00001706926 fastq.gz 785.2 MB
EGAF00001706927 fastq.gz 3.9 GB
EGAF00001706928 fastq.gz 694.7 MB
EGAF00001706929 fastq.gz 3.3 GB
EGAF00001706930 fastq.gz 704.1 MB
EGAF00001706931 fastq.gz 3.3 GB
EGAF00001706932 fastq.gz 708.2 MB
EGAF00001706933 fastq.gz 3.4 GB
EGAF00001706934 fastq.gz 695.7 MB
EGAF00001706935 fastq.gz 3.3 GB
EGAF00001706936 fastq.gz 701.3 MB
EGAF00001706937 fastq.gz 3.4 GB
EGAF00001706938 fastq.gz 679.4 MB
EGAF00001706939 fastq.gz 3.3 GB
EGAF00001706940 fastq.gz 686.6 MB
EGAF00001706941 fastq.gz 3.3 GB
EGAF00001706942 fastq.gz 708.3 MB
EGAF00001706943 fastq.gz 3.5 GB
EGAF00001706944 fastq.gz 767.5 MB
EGAF00001706945 fastq.gz 3.6 GB
EGAF00001706946 fastq.gz 777.7 MB
EGAF00001706947 fastq.gz 3.7 GB
EGAF00001706948 fastq.gz 783.3 MB
EGAF00001706949 fastq.gz 3.8 GB
EGAF00001706950 fastq.gz 769.2 MB
EGAF00001706951 fastq.gz 3.7 GB
EGAF00001706952 fastq.gz 776.8 MB
EGAF00001706953 fastq.gz 3.8 GB
EGAF00001706954 fastq.gz 751.1 MB
EGAF00001706955 fastq.gz 3.6 GB
EGAF00001706956 fastq.gz 759.3 MB
EGAF00001706957 fastq.gz 3.7 GB
EGAF00001706958 fastq.gz 784.2 MB
EGAF00001706959 fastq.gz 3.9 GB
EGAF00001706960 fastq.gz 661.6 MB
EGAF00001706961 fastq.gz 3.1 GB
EGAF00001706962 fastq.gz 671.6 MB
EGAF00001706963 fastq.gz 3.2 GB
EGAF00001706964 fastq.gz 676.1 MB
EGAF00001706965 fastq.gz 3.3 GB
EGAF00001706966 fastq.gz 663.0 MB
EGAF00001706967 fastq.gz 3.2 GB
EGAF00001706968 fastq.gz 668.9 MB
EGAF00001706969 fastq.gz 3.2 GB
EGAF00001706970 fastq.gz 647.0 MB
EGAF00001706971 fastq.gz 3.1 GB
EGAF00001706972 fastq.gz 653.2 MB
EGAF00001706973 fastq.gz 3.2 GB
EGAF00001706974 fastq.gz 675.8 MB
EGAF00001706975 fastq.gz 3.3 GB
EGAF00001706976 fastq.gz 683.6 MB
EGAF00001706977 fastq.gz 3.2 GB
EGAF00001706978 fastq.gz 693.0 MB
EGAF00001706979 fastq.gz 3.3 GB
EGAF00001706980 fastq.gz 697.6 MB
EGAF00001706981 fastq.gz 3.4 GB
EGAF00001706982 fastq.gz 684.7 MB
EGAF00001706983 fastq.gz 3.3 GB
EGAF00001706984 fastq.gz 690.7 MB
EGAF00001706985 fastq.gz 3.3 GB
EGAF00001706986 fastq.gz 668.1 MB
EGAF00001706987 fastq.gz 3.2 GB
EGAF00001706988 fastq.gz 675.2 MB
EGAF00001706989 fastq.gz 3.3 GB
EGAF00001706990 fastq.gz 697.7 MB
EGAF00001706991 fastq.gz 3.4 GB
EGAF00001706992 fastq.gz 740.7 MB
EGAF00001706993 fastq.gz 3.4 GB
EGAF00001706994 fastq.gz 731.3 MB
EGAF00001706995 fastq.gz 3.4 GB
EGAF00001706996 fastq.gz 730.0 MB
EGAF00001706997 fastq.gz 3.4 GB
EGAF00001706998 fastq.gz 747.5 MB
EGAF00001706999 fastq.gz 3.5 GB
EGAF00001707000 fastq.gz 755.5 MB
EGAF00001707001 fastq.gz 3.5 GB
EGAF00001707002 fastq.gz 706.1 MB
EGAF00001707003 fastq.gz 3.3 GB
EGAF00001707004 fastq.gz 678.3 MB
EGAF00001707005 fastq.gz 3.2 GB
EGAF00001707006 fastq.gz 731.8 MB
EGAF00001707007 fastq.gz 3.5 GB
136 Files (525.3 GB)