SCLC study MGH - WES dataset

Dataset ID Technology Samples
EGAD00001003970 Illumina HiSeq 2000 7

Dataset Description

For whole-exome sequencing 1 µg of DNA from fresh-frozen tumors was fragmented by sonication technology (for DNA from fresh-frozen tumors: Bioruptor, diagenode, Liѐge, Belgium; for DNA from FFPE material: Covaris). The fragments were end-repaired and adaptor-ligated, including incorporation of sample index barcodes. After size selection, libraries were subjected to an enrichment process with Sure select XT (Agilent). The final libraries were sequenced with a paired-end 2×75 bp protocol for an average coverage of 100-120x

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

DAC University of Cologne
Contact person: Dr. Graziella Bosco
Email: dac-info [at] uni-koeln [dot] de
More details: EGAC00001000064


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