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NGS data of human NES cells and tumors

Raw RNA-seq data for WT and 2 Gorlin NES cells, tumors derived from MYCN mis-expressed WT NES cells, tumors derived from Gorlin NES cells, and tumors derived from Gorlin NES cells transduced with mutant DDX3X (R351W and R534S) and CRISPR/Cas9 targeting GSE1 (each sample has 3 replicates/tumors except Gorlin NES cell tumors have 4). Raw whole exome sequencing data for WT and Gorlin 1 NES cells, tumors derived from MYCN mis-expressed WT NES cells, and tumors derived from Gorlin NES cells (each sample has 3 replicates/tumors except Gorlin NES cell tumors have 4). Raw data for amplicon sequencing of GSE1 and KDM3B at regions targeted by CRISPR/Cas9 in Gorlin NES cells.

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RNA-seq and whole exome sequencing data on tumors derived from human NES cells. Also includes amplicon sequencing of GSE1 and KDM3B in human NES cells transduced with CRISPR/Cas9 targeting each gene

DATA ACCESS AGREEMENT These terms and conditions govern access to the managed access datasets (details of which are set out in Appendix I) to which the User Institution has requested access. The User Institution agrees to be bound by these terms and conditions. Definitions Authorised Personnel: The individuals at the User Institution to whom UCSF grants access to the Data. This includes the User, the individuals listed in Appendix II and any other individuals for whom the User Institution subsequently requests access to the Data. Details of the initial Authorised Personnel are set out in Appendix II. Data: The managed access datasets to which the User Institution has requested access. Data Producers: UCSF and the collaborators listed in Appendix I responsible for the development, organisation, and oversight of these Data. External Collaborator: A collaborator of the User, working for an institution other than the User Institution. Project: The project for which the User Institution has requested access to these Data. A description of the Project is set out in Appendix II. Publications: Includes, without limitation, articles published in print journals, electronic journals, reviews, books, posters and other written and verbal presentations of research. Research Participant: An individual whose data form part of these Data. Research Purposes: Shall mean research that is seeking to advance the understanding of genetics and genomics, including the treatment of disorders, and work on statistical methods that may be applied to such research. User: The principal investigator for the Project. User Institution(s): The Institution that has requested access to the Data. XXXXX: Your Institution details here 1. The User Institution agrees to only use these Data for the purpose of the Project (described in Appendix II) and only for Research Purposes. The User Institution further agrees that it will only use these Data for Research Purposes which are within the limitations (if any) set out in Appendix I. 2. The User Institution agrees to preserve, at all times, the confidentiality of these Data. In particular, it undertakes not to use, or attempt to use these Data to compromise or otherwise infringe the confidentiality of information on Research Participants. Without prejudice to the generality of the foregoing, the User Institution agrees to use at least the measures set out in Appendix I to protect these Data. 3. The User Institution agrees to protect the confidentiality of Research Participants in any research papers or publications that they prepare by taking all reasonable care to limit the possibility of identification. 4. The User Institution agrees not to link or combine these Data to other information or archived data available in a way that could re-identify the Research Participants, even if access to that data has been formally granted to the User Institution or is freely available without restriction. 5. The User Institution agrees only to transfer or disclose these Data, in whole or part, or any material derived from these Data, to the Authorised Personnel. Should the User Institution wish to share these Data with an External Collaborator, the External Collaborator must complete a separate application for access to these Data. 6. The User Institution agrees that the Data Producers, and all other parties involved in the creation, funding or protection of these Data: a) make no warranty or representation, express or implied as to the accuracy, quality or comprehensiveness of these Data; b) exclude to the fullest extent permitted by law all liability for actions, claims, proceedings, demands, losses (including but not limited to loss of profit), costs, awards damages and payments made by the Recipient that may arise (whether directly or indirectly) in any way whatsoever from the Recipient’s use of these Data or from the unavailability of, or break in access to, these Data for whatever reason and; c) bear no responsibility for the further analysis or interpretation of these Data. 7. The User Institution agrees to follow the Fort Lauderdale Guidelines (http://www.wellcome.ac.uk/stellent/groups/corporatesite/@policy_communications/documents/web_document/wtd003207.pdf ) and the Toronto Statement (http://www.nature.com/nature/journal/v461/n7261/full/461168a.html). This includes but is not limited to recognising the contribution of the Data Producers and including a proper acknowledgement in all reports or publications resulting from the use of these Data. 8. The User Institution agrees to follow the Publication Policy in Appendix III. This includes respecting the moratorium period for the Data Producers to publish the first peer-reviewed report describing and analysing these Data. 9. The User Institution agrees not to make intellectual property claims on these Data and not to use intellectual property protection in ways that would prevent or block access to, or use of, any element of these Data, or conclusion drawn directly from these Data. 10. The User Institution can elect to perform further research that would add intellectual and resource capital to these data and decide to obtain intellectual property rights on these downstream discoveries. In this case, the User Institution agrees to implement licensing policies that will not obstruct further research and to follow the U.S. National Institutes of Health Best Practices for the Licensing of Genomic Inventions (2005) (https://www.icgc.org/files/daco/NIH_BestPracticesLicensingGenomicInventions_2005_en.pdf ) in conformity with the Organisation for Economic Co-operation and Development Guidelines for the Licensing of the Genetic Inventions (2006) (http://www.oecd.org/science/biotech/36198812.pdf ). 11. The User Institution agrees to destroy/discard the Data held, once it is no longer used for the Project, unless obliged to retain the data for archival purposes in conformity with audit or legal requirements. 12. The User Institution will notify UCSF within 30 days of any changes or departures of Authorised Personnel. 13. The User Institution will notify UCSF prior to any significant changes to the protocol for the Project. 14. The User Institution will notify UCSF as soon as it becomes aware of a breach of the terms or conditions of this agreement. 15. UCSF may terminate this agreement by written notice to the User Institution. If this agreement terminates for any reason, the User Institution will be required to destroy any Data held, including copies and backup copies. This clause does not prevent the User Institution from retaining these data for archival purpose in conformity with audit or legal requirements. 16. The User Institution accepts that it may be necessary for the Data Producers to alter the terms of this agreement from time to time. As an example, this may include specific provisions relating to the Data required by Data Producers other than UCSF. In the event that changes are required, the Data Producers or their appointed agent will contact the User Institution to inform it of the changes and the User Institution may elect to accept the changes or terminate the agreement. 17. If requested, the User Institution will allow data security and management documentation to be inspected to verify that it is complying with the terms of this agreement. 18. The User Institution agrees to distribute a copy of these terms to the Authorised Personnel. The User Institution will procure that the Authorised Personnel comply with the terms of this agreement. 19. This agreement (and any dispute, controversy, proceedings or claim of whatever nature arising out of this agreement or its formation) shall be construed, interpreted and governed by the laws of the United States of America (USA) and shall be subject to the exclusive jurisdiction of the USA courts. Agreed for User Institution Signature: Name: Title: Date: Principal Investigator I confirm that I have read and understood this Agreement. Signature: Name: Title: Date: Agreed for UCSF Signature: Name: Title: Date: APPENDIX I – DATASET DETAILS APPENDIX II ––PROJECT DETAILS APPENDIX III –– PUBLICATION POLICY APPENDIX I – DATASET DETAILS (to be completed by the data producer before passing to applicant) Dataset reference (EGA Study ID and Dataset Details) EGAS00001003529 Name of project that created the dataset Recapitulation of genetic predisposition to medulloblastoma in human neuroepithelial stem cells Names of other data producers/collaborators Specific limitations on areas of research Minimum protection measures required File access: Data can be held in unencrypted files on an institutional compute system, with Unix user group read/write access for one or more appropriate groups but not Unix world read/write access behind a secure firewall. Laptops holding these data should have password protected logins and screenlocks (set to lock after 5 min of inactivity). If held on USB keys or other portable hard drives, the data must be encrypted. APPENDIX II – PROJECT DETAILS (to be completed by the Requestor) Details of dataset requested i.e., EGA Study and Dataset Accession Number Brief abstract of the Project in which the Data will be used (500 words max) All Individuals who the User Institution to be named as registered users Name of Registered User Email Job Title Supervisor* All Individuals that should have an account created at the EGA Name of Registered User Email Job Title APPENDIX III – PUBLICATION POLICY UCSF intend to publish the results of their analysis of this dataset and do not consider its deposition into public databases to be the equivalent of such publications. UCSF anticipate that the dataset could be useful to other qualified researchers for a variety of purposes. However, some areas of work are subject to a publication moratorium. The publication moratorium covers any publications (including oral communications) that describe the use of the dataset. For research papers, submission for publication should not occur until 2 months after these data were first made available on the relevant hosting database, unless UCSF has provided written consent to earlier submission. In any publications based on these data, please describe how the data can be accessed, including the name of the hosting database (e.g., The European Genome-phenome Archive at the European Bioinformatics Institute) and its accession numbers (e.g., EGAS00000000029), and acknowledge its use in a form agreed by the User Institution with UCSF.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001003620 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00002454806 fq.gz 3.2 GB
EGAF00002454807 fq.gz 3.3 GB
EGAF00002454808 fq.gz 3.8 GB
EGAF00002454809 fq.gz 3.9 GB
EGAF00002454810 fq.gz 3.9 GB
EGAF00002454811 fq.gz 4.0 GB
EGAF00002454812 fq.gz 3.7 GB
EGAF00002454813 fq.gz 3.8 GB
EGAF00002454814 fq.gz 6.4 GB
EGAF00002454815 fq.gz 6.6 GB
EGAF00002454816 fq.gz 6.3 GB
EGAF00002454817 fq.gz 6.4 GB
EGAF00002454818 fastq.gz 10.6 GB
EGAF00002454819 fastq.gz 10.7 GB
EGAF00002454820 fastq.gz 10.7 GB
EGAF00002454821 fastq.gz 11.0 GB
EGAF00002454822 fastq.gz 10.5 GB
EGAF00002454823 fastq.gz 10.7 GB
EGAF00002454824 fastq.gz 9.4 GB
EGAF00002454825 fastq.gz 9.5 GB
EGAF00002454826 fq.gz 5.0 GB
EGAF00002454827 fq.gz 5.3 GB
EGAF00002454828 fq.gz 4.8 GB
EGAF00002454829 fq.gz 5.2 GB
EGAF00002454830 fq.gz 4.7 GB
EGAF00002454831 fq.gz 5.1 GB
EGAF00002454832 fq.gz 5.0 GB
EGAF00002454833 fq.gz 5.3 GB
EGAF00002454834 fq.gz 7.8 GB
EGAF00002454835 fq.gz 8.3 GB
EGAF00002454836 fq.gz 7.7 GB
EGAF00002454837 fq.gz 8.1 GB
EGAF00002454838 fq.gz 9.5 GB
EGAF00002454839 fq.gz 10.2 GB
EGAF00002454840 fq.gz 10.8 GB
EGAF00002454841 fq.gz 11.3 GB
EGAF00002454842 fq.gz 10.4 GB
EGAF00002454843 fq.gz 11.0 GB
EGAF00002454844 fq.gz 4.7 GB
EGAF00002454845 fq.gz 5.0 GB
EGAF00002454846 fq.gz 6.1 GB
EGAF00002454847 fq.gz 6.5 GB
EGAF00002454848 fq.gz 5.3 GB
EGAF00002454849 fq.gz 5.7 GB
EGAF00002454850 fq.gz 5.6 GB
EGAF00002454851 fq.gz 6.0 GB
EGAF00002454852 fq.gz 6.1 GB
EGAF00002454853 fq.gz 6.5 GB
EGAF00002454854 fq.gz 5.5 GB
EGAF00002454855 fq.gz 6.0 GB
EGAF00002454856 fq.gz 5.5 GB
EGAF00002454857 fq.gz 5.9 GB
EGAF00002454858 fq.gz 3.3 GB
EGAF00002454859 fq.gz 3.6 GB
EGAF00002454860 fq.gz 442.1 MB
EGAF00002454861 fq.gz 481.4 MB
EGAF00002454862 fq.gz 4.6 GB
EGAF00002454863 fq.gz 4.7 GB
EGAF00002454864 fq.gz 3.4 GB
EGAF00002454865 fq.gz 3.8 GB
EGAF00002454866 fq.gz 461.3 MB
EGAF00002454867 fq.gz 500.7 MB
EGAF00002454868 fq.gz 4.1 GB
EGAF00002454869 fq.gz 4.3 GB
EGAF00002454870 fq.gz 621.5 MB
EGAF00002454871 fq.gz 664.3 MB
EGAF00002454872 fq.gz 4.2 GB
EGAF00002454873 fq.gz 4.4 GB
EGAF00002454874 fq.gz 628.5 MB
EGAF00002454875 fq.gz 675.7 MB
EGAF00002454876 fq.gz 5.2 GB
EGAF00002454877 fq.gz 5.4 GB
EGAF00002454878 fq.gz 776.1 MB
EGAF00002454879 fq.gz 821.4 MB
EGAF00002454880 fq.gz 2.9 GB
EGAF00002454881 fq.gz 3.0 GB
EGAF00002454882 fq.gz 429.9 MB
EGAF00002454883 fq.gz 460.1 MB
EGAF00002454884 fq.gz 4.1 GB
EGAF00002454885 fq.gz 4.2 GB
EGAF00002454886 fq.gz 3.1 GB
EGAF00002454887 fq.gz 3.4 GB
EGAF00002454888 fq.gz 417.3 MB
EGAF00002454889 fq.gz 451.6 MB
EGAF00002454890 fq.gz 3.4 GB
EGAF00002454891 fq.gz 3.5 GB
EGAF00002454892 fq.gz 3.9 GB
EGAF00002454893 fq.gz 4.5 GB
EGAF00002454894 fq.gz 4.5 GB
EGAF00002454895 fq.gz 5.2 GB
EGAF00002454896 fq.gz 2.4 GB
EGAF00002454897 fq.gz 2.8 GB
EGAF00002454898 fq.gz 1.6 GB
EGAF00002454899 fq.gz 1.7 GB
EGAF00002458262 fastq.gz 12.3 MB
EGAF00002458263 fastq.gz 15.8 MB
EGAF00002458264 fastq.gz 15.7 MB
EGAF00002458265 fastq.gz 22.4 MB
98 Files (467.0 GB)