Dataset

Whole Genome Sequencing of CRLF2/IL7RA transduced cells

Dataset ID Technology Samples
EGAD00001005456 Illumina HiSeq 2500 2

Dataset Description

There are two samples, 42 (control) and 49
To test the role of activated CRLF2/IL7RA in leukemia initiation we expressed CRLF2 together with IL7RA in human CB hematopoietic progenitors. Human CRLF2 and wild type and/or activated mutant form of human IL7RA (IL7RAwt/ins) were cloned into a lentiviral vector with a bi-cistronic cassette under the expression control of an Eμ-B29 promoter/enhancer to augment expression in B-cell precursors. Backbone vector expressing GFP (BB).
Whole genome sequencing
Leukemic (49) and BB transduced (42) corresponding CB cells were collected from transplanted mice. Sequencing libraries were prepared from these samples

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

UCL Cancer Institute Data Access Committee
Contact person: Tariq Enver
Email: t [dot] enver [at] ucl [dot] ac [dot] uk
More details: EGAC00001001360

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