Spatial RNA-sequencing of metastatic melanoma

Dataset ID Technology Samples
EGAD00001005820 Illumina HiSeq 2500 48

Dataset Description

We performed RNA sequencing of 48 different regions sub-sampled from a metastatic melanoma biopsy that was treated with anti-PD-1 inhibitor. RNAseq was performed on samples with a minimum RNA integrity number (RIN) of 5.5 except for two cases (6A10 and 8A3) with RINs greater than 3. A minimum of 700ng of RNA were required for all samples undergoing RNAseq. Paired-end transcriptome reads were aligned using TopHat2, to the UCSC hg19 reference genome.

Data Use Conditions


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Label Code Version Modifier
obsolete research use only DUO:0000014 2019-01-07