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Dataset

Spatial RNA-sequencing of metastatic melanoma

Dataset ID	Technology	Samples
EGAD00001005820	Illumina HiSeq 2500	48

Dataset Description

We performed RNA sequencing of 48 different regions sub-sampled from a metastatic melanoma biopsy that was treated with anti-PD-1 inhibitor. RNAseq was performed on samples with a minimum RNA integrity number (RIN) of 5.5 except for two cases (6A10 and 8A3) with RINs greater than 3. A minimum of 700ng of RNA were required for all samples undergoing RNAseq. Paired-end transcriptome reads were aligned using TopHat2, to the UCSC hg19 reference genome.

Data Use Conditions

RUO

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Label	Code	Version	Modifier
obsolete research use only	DUO:0000014	2019-01-07

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

Melanoma Spatial Heterogeneity Data Access Committee
Contact person: Andrew Futreal
Email: afutreal [at] mdanderson [dot] org
More details: EGAC00001001446

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This dataset is featured in 1 study

Studies are experimental investigations of a particular phenomenon. e.g. case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients. Click on one of the Study IDs below to find out more.

Study ID	Study Title	Study Type
EGAS00001003292	Whole Tumor spatial heterogeneity in metastatic melanoma	Other

EGA Beacon