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Whole exome sequencing data from non-small-cell lung cancer patients receiving immunotherapy prognosis

The plasma samples and white blood cell samples were collected from 30 non-small-cell lung cancer patients and 3 healthy individuals. The solid tumor biopsy samples from 14 patients (a subset of the 30 patients) were collected. The cfDNA was extracted from their plasma samples using the QIAamp circulating nucleic acid kit from QIAGEN (Germantown, MD). The cfDNA WES library was constructed with the SureSelect XT HS kit from Agilent Technologies (Santa Clara, CA) according to the manufacturer’s protocol. In brief, 10ng of cfDNA was used as input material. After end repair/dA-tailing of cfDNA, the adaptor was ligated. The ligation product was purified with Ampure XP beads (Beckman-Coulter, Atlanta, GA) and the adaptor-ligated library was amplified with index primer in 10-cycle PCR. The amplified library was purified again with Ampure XP beads, and the amount of amplified DNA was measured using the Qubit 1xdsDNA HS assay kit (ThermoFisher, Waltham, MA). 700-1000 ng of DNA sample was hybridized to the Agilent SureSelect Human All Exon V6 (Agilent) capture library and pulled down by streptavidin-coated beads. After washing the beads, the DNA library captured on the beads was re-amplified with 10-cycle PCR. The final libraries were purified by Ampure XP beads. The library concentration was measured by Qubit, and the quality was further examined with Agilent Bioanalyzer before the final step of 2x150bp paired-end sequencing on the Illumina HiSeq X10 platform (Illumina) at an average coverage of 200. Whole-exome capture libraries of genomic DNA of the 30 non-small cell lung cancer patients were constructed via Roche SeqCap EZ Exome V3 (Roche); whole-exome capture libraries of genomic DNA of the 3 healthy individuals were constructed via Agilent SureSelect Human All Exon V6 (Agilent). Enriched exome libraries were sequenced on the Illumina HiSeq 3000 platform (Illumina) to generate 2x100bp paired-end reads at an average coverage of 200.

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The data usage policy for whole-exome sequencing data of cell-free DNA derived from non-small-cell lung cancer patients receiving immunotherapies

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001004373 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00003620566 fastq.gz 6.0 GB
EGAF00003620567 fastq.gz 6.7 GB
EGAF00003620568 fastq.gz 7.2 GB
EGAF00003620569 fastq.gz 7.9 GB
EGAF00003620570 fastq.gz 7.3 GB
EGAF00003620571 fastq.gz 8.0 GB
EGAF00003620572 fastq.gz 8.3 GB
EGAF00003620573 fastq.gz 9.2 GB
EGAF00003620574 fastq.gz 8.2 GB
EGAF00003620575 fastq.gz 9.1 GB
EGAF00003620576 fastq.gz 7.5 GB
EGAF00003620577 fastq.gz 8.3 GB
EGAF00003620578 fastq.gz 7.0 GB
EGAF00003620579 fastq.gz 7.7 GB
EGAF00003620580 fastq.gz 7.5 GB
EGAF00003620581 fastq.gz 8.3 GB
EGAF00003620582 fastq.gz 4.9 GB
EGAF00003620583 fastq.gz 5.4 GB
EGAF00003620584 fastq.gz 9.9 GB
EGAF00003620585 fastq.gz 10.7 GB
EGAF00003620586 fastq.gz 6.1 GB
EGAF00003620587 fastq.gz 6.6 GB
EGAF00003620588 fastq.gz 8.4 GB
EGAF00003620589 fastq.gz 9.0 GB
EGAF00003620590 fastq.gz 8.3 GB
EGAF00003620591 fastq.gz 8.9 GB
EGAF00003620592 fastq.gz 4.6 GB
EGAF00003620593 fastq.gz 4.9 GB
EGAF00003620594 fastq.gz 2.7 GB
EGAF00003620595 fastq.gz 2.9 GB
EGAF00003620596 fastq.gz 10.2 GB
EGAF00003620597 fastq.gz 11.0 GB
EGAF00003620598 fastq.gz 6.2 GB
EGAF00003620599 fastq.gz 6.6 GB
EGAF00003620600 fastq.gz 9.0 GB
EGAF00003620601 fastq.gz 9.5 GB
EGAF00003620602 fastq.gz 7.9 GB
EGAF00003620603 fastq.gz 8.3 GB
EGAF00003620604 fastq.gz 10.6 GB
EGAF00003620605 fastq.gz 11.3 GB
EGAF00003620606 fastq.gz 7.2 GB
EGAF00003620607 fastq.gz 7.7 GB
EGAF00003620608 fastq.gz 8.1 GB
EGAF00003620609 fastq.gz 8.6 GB
EGAF00003620610 fastq.gz 7.9 GB
EGAF00003620611 fastq.gz 8.4 GB
EGAF00003620612 fastq.gz 7.6 GB
EGAF00003620613 fastq.gz 8.1 GB
EGAF00003620614 fastq.gz 8.5 GB
EGAF00003620615 fastq.gz 9.0 GB
EGAF00003620616 fastq.gz 9.9 GB
EGAF00003620617 fastq.gz 10.6 GB
EGAF00003620618 fastq.gz 8.6 GB
EGAF00003620619 fastq.gz 9.2 GB
EGAF00003620620 fastq.gz 8.4 GB
EGAF00003620621 fastq.gz 9.0 GB
EGAF00003620622 fastq.gz 9.6 GB
EGAF00003620623 fastq.gz 10.2 GB
EGAF00003620624 fastq.gz 6.6 GB
EGAF00003620625 fastq.gz 7.0 GB
EGAF00003620626 fastq.gz 7.0 GB
EGAF00003620627 fastq.gz 7.8 GB
EGAF00003620628 fastq.gz 5.8 GB
EGAF00003620629 fastq.gz 6.2 GB
EGAF00003620630 fastq.gz 6.7 GB
EGAF00003620631 fastq.gz 6.8 GB
EGAF00003620632 fastq.gz 8.8 GB
EGAF00003620633 fastq.gz 10.0 GB
EGAF00003620634 fastq.gz 11.9 GB
EGAF00003620635 fastq.gz 13.3 GB
EGAF00003620636 fastq.gz 12.0 GB
EGAF00003620637 fastq.gz 13.5 GB
EGAF00003620638 fastq.gz 12.5 GB
EGAF00003620639 fastq.gz 14.3 GB
EGAF00003620640 fastq.gz 7.4 GB
EGAF00003620641 fastq.gz 7.9 GB
EGAF00003620642 fastq.gz 10.8 GB
EGAF00003620643 fastq.gz 12.2 GB
EGAF00003620644 fastq.gz 13.5 GB
EGAF00003620645 fastq.gz 15.6 GB
EGAF00003620646 fastq.gz 8.4 GB
EGAF00003620647 fastq.gz 8.3 GB
EGAF00003620648 fastq.gz 9.4 GB
EGAF00003620649 fastq.gz 9.4 GB
EGAF00003620650 fastq.gz 7.3 GB
EGAF00003620651 fastq.gz 8.5 GB
EGAF00003620652 fastq.gz 8.6 GB
EGAF00003620653 fastq.gz 8.6 GB
EGAF00003620654 fastq.gz 6.3 GB
EGAF00003620655 fastq.gz 7.1 GB
EGAF00003620656 fastq.gz 12.5 GB
EGAF00003620657 fastq.gz 13.9 GB
EGAF00003620658 fastq.gz 14.4 GB
EGAF00003620659 fastq.gz 16.5 GB
EGAF00003620660 fastq.gz 12.2 GB
EGAF00003620661 fastq.gz 13.6 GB
EGAF00003620662 fastq.gz 7.0 GB
EGAF00003620663 fastq.gz 7.3 GB
EGAF00003620664 fastq.gz 5.6 GB
EGAF00003620665 fastq.gz 5.6 GB
EGAF00003620666 fastq.gz 5.2 GB
EGAF00003620667 fastq.gz 5.2 GB
EGAF00003620668 fastq.gz 8.8 GB
EGAF00003620669 fastq.gz 8.8 GB
EGAF00003620670 fastq.gz 7.3 GB
EGAF00003620671 fastq.gz 7.3 GB
EGAF00003620672 fastq.gz 8.2 GB
EGAF00003620673 fastq.gz 8.2 GB
EGAF00003620674 fastq.gz 4.9 GB
EGAF00003620675 fastq.gz 5.1 GB
EGAF00003620676 fastq.gz 8.4 GB
EGAF00003620677 fastq.gz 9.1 GB
EGAF00003620678 fastq.gz 6.3 GB
EGAF00003620679 fastq.gz 6.3 GB
EGAF00003620680 fastq.gz 6.0 GB
EGAF00003620681 fastq.gz 6.0 GB
EGAF00003620682 fastq.gz 6.6 GB
EGAF00003620683 fastq.gz 6.6 GB
EGAF00003620684 fastq.gz 8.7 GB
EGAF00003620685 fastq.gz 9.4 GB
EGAF00003620686 fastq.gz 11.3 GB
EGAF00003620687 fastq.gz 12.4 GB
EGAF00003620688 fastq.gz 10.0 GB
EGAF00003620689 fastq.gz 9.9 GB
EGAF00003620690 fastq.gz 14.4 GB
EGAF00003620691 fastq.gz 14.4 GB
EGAF00003620692 fastq.gz 11.0 GB
EGAF00003620693 fastq.gz 11.0 GB
EGAF00003620694 fastq.gz 7.0 GB
EGAF00003620695 fastq.gz 6.9 GB
EGAF00003620696 fastq.gz 9.3 GB
EGAF00003620697 fastq.gz 10.3 GB
EGAF00003620698 fastq.gz 4.8 GB
EGAF00003620699 fastq.gz 4.7 GB
EGAF00003620700 fastq.gz 11.9 GB
EGAF00003620701 fastq.gz 11.9 GB
EGAF00003620702 fastq.gz 12.8 GB
EGAF00003620703 fastq.gz 12.8 GB
EGAF00003620704 fastq.gz 4.7 GB
EGAF00003620705 fastq.gz 4.7 GB
EGAF00003620706 fastq.gz 6.2 GB
EGAF00003620707 fastq.gz 6.2 GB
EGAF00003620708 fastq.gz 5.3 GB
EGAF00003620709 fastq.gz 5.3 GB
EGAF00003620710 fastq.gz 8.5 GB
EGAF00003620711 fastq.gz 8.5 GB
EGAF00003620712 fastq.gz 8.5 GB
EGAF00003620713 fastq.gz 9.4 GB
EGAF00005186875 fastq.gz 7.2 GB
EGAF00005186876 fastq.gz 8.2 GB
EGAF00005186877 fastq.gz 6.7 GB
EGAF00005186878 fastq.gz 7.6 GB
EGAF00005186879 fastq.gz 6.7 GB
EGAF00005186880 fastq.gz 7.7 GB
EGAF00005186881 fastq.gz 7.9 GB
EGAF00005186882 fastq.gz 8.9 GB
EGAF00005186883 fastq.gz 7.0 GB
EGAF00005186884 fastq.gz 8.1 GB
EGAF00005186885 fastq.gz 6.6 GB
EGAF00005186886 fastq.gz 7.6 GB
EGAF00005186887 fastq.gz 6.8 GB
EGAF00005186888 fastq.gz 7.6 GB
EGAF00005186889 fastq.gz 6.5 GB
EGAF00005186890 fastq.gz 7.4 GB
EGAF00005186891 fastq.gz 6.9 GB
EGAF00005186892 fastq.gz 7.8 GB
166 Files (1.4 TB)