About file location

As the EGA is co-managed by the EMBL-EBI and CRG, files could be located in the UK and/or Spain, as indicated by their respective flags.

Alternatively, files could be located in one of the international Federated EGA nodes, as indicated next to the node's flag.
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RNA-seq study of a Princess Margaret Cancer Centre human acute myeloid leukemia patient cohort

The PMCC AML RNAseq dataset consists of 81 AML patient samples (clinical data in Supplemental Table 11 of manuscript), processed in two batches. These patient samples are able to engraft in the NSG (NOD.Cg PrkdcscidIl2rgtm1Wjl /SzJ) mouse model. Five patients (90543, 598, 90240, 110484, 100500) were included in both batches. Viaably frozen material from the Leukemia Tissue Bank at Princess Margaret Cancer Centre/ University Health Network were thawed by dropwise addition of X-VIVO + 50% fetal calf serum supplemented with DNase (100μg/mL final concentration, Roche). RNA was extracted from bulk peripheral blood mononuclear cells (PBMC) using the RNeasy Micro Kit (Qiagen Inc.). A paired-end 76 base-pair flow-cell lane Illumina High seq 2000 yielded an average of 240 million sequence reads aligning to genome per sample at the Genome Sciences Centre, BC Cancer Agency for cohort 1. Cohort 2 was subjected to 125 bp, paired-end RNA-sequencing on the Illumina HiSeq 2500 with an average of 50 million reads/sample at the Centre for Applied Genomics, Sick Kids Hospital.

Request Access

Access to data generated by UHN is made available by completing the data access agreement for review by the data access committee and will be granted to qualified investigators for appropriate use.

DATA ACCESS AGREEMENT These terms and conditions govern access to the managed access datasets (details of which are set out in Appendix I) to which the User Institution has requested access. The User Institution agrees to be bound by these terms and conditions. Definitions Authorised Personnel: The individuals at the User Institution to whom the Data Access Committee (DAC) grants access to the Data. This includes the User, the individuals listed in Appendix II and any other individuals for whom the User Institution subsequently requests access to the Data. Details of the initial Authorised Personnel are set out in Appendix II. Data: The managed access datasets to which the User Institution has requested access. Data Producers: The Principal Investigator and the collaborators listed in Appendix I responsible for the development, organisation, and oversight of these Data. External Collaborator: A collaborator of the User, working for an institution other than the User Institution. Project: The project for which the User Institution has requested access to these Data. A description of the Project is set out in Appendix II. Publications: Includes, without limitation, articles published in print journals, electronic journals, reviews, books, posters and other written and verbal presentations of research. Research Participant: An individual whose data form part of these Data. Research Purposes: Shall mean research that is seeking to advance the understanding of genetics and genomics, including the treatment of disorders, and work on statistical methods that may be applied to such research. User: The principal investigator for the Project. User Institution(s): The Institution that has requested access to the Data. University Health Network: The University Health Network (UHN) is the largest clinical and research hospital in Canada. UHN is affiliated with the University of Toronto and is a member of the Toronto Academic Health Science Network. 1. The User Institution agrees to only use these Data for the purpose of the Project (described in Appendix II) and only for Research Purposes. The User Institution further agrees that it will only use these Data for Research Purposes which are within the limitations (if any) set out in Appendix I. 2. The User Institution agrees to preserve, at all times, the confidentiality of these Data. In particular, it undertakes not to use, or attempt to use these Data to compromise or otherwise infringe the confidentiality of information on Research Participants. Without prejudice to the generality of the foregoing, the User Institution agrees to use at least the measures set out in Appendix I to protect these Data. 3. The User Institution agrees to protect the confidentiality of Research Participants in any research papers or publications that they prepare by taking all reasonable care to limit the possibility of identification. 4. The User Institution agrees not to link or combine these Data to other information or archived data available in a way that could re-identify the Research Participants, even if access to that data has been formally granted to the User Institution or is freely available without restriction. 5. The User Institution agrees only to transfer or disclose these Data, in whole or part, or any material derived from these Data, to the Authorised Personnel. Should the User Institution wish to share these Data with an External Collaborator, the External Collaborator must complete a separate application for access to these Data. 6. The User Institution agrees that the Data Producers, and all other parties involved in the creation, funding or protection of these Data: a) make no warranty or representation, express or implied as to the accuracy, quality or comprehensiveness of these Data; b) exclude to the fullest extent permitted by law all liability for actions, claims, proceedings, demands, losses (including but not limited to loss of profit), costs, awards damages and payments made by the Recipient that may arise (whether directly or indirectly) in any way whatsoever from the Recipient’s use of these Data or from the unavailability of, or break in access to, these Data for whatever reason and; c) bear no responsibility for the further analysis or interpretation of these Data. 7. The User Institution agrees to follow the Fort Lauderdale Guidelines (http://www.wellcome.ac.uk/stellent/groups/corporatesite/@policy_communications/documents/web_document/wtd003207.pdf ) and the Toronto Statement (http://www.nature.com/nature/journal/v461/n7261/full/461168a.html). This includes but is not limited to recognising the contribution of the Data Producers and including a proper acknowledgement in all reports or publications resulting from the use of these Data. 8. The User Institution agrees to follow the Publication Policy in Appendix III. This includes respecting the moratorium period for the Data Producers to publish the first peer-reviewed report describing and analysing these Data. 9. The User Institution agrees not to make intellectual property claims on these Data and not to use intellectual property protection in ways that would prevent or block access to, or use of, any element of these Data, or conclusion drawn directly from these Data. 10. The User Institution can elect to perform further research that would add intellectual and resource capital to these data and decide to obtain intellectual property rights on these downstream discoveries. In this case, the User Institution agrees to implement licensing policies that will not obstruct further research and to follow the U.S. National Institutes of Health Best Practices for the Licensing of Genomic Inventions (2005) (https://www.icgc.org/files/daco/NIH_BestPracticesLicensingGenomicInventions_2005_en.pdf ) in conformity with the Organisation for Economic Co-operation and Development Guidelines for the Licensing of the Genetic Inventions (2006) (http://www.oecd.org/science/biotech/36198812.pdf ). 11. The User Institution agrees to destroy/discard the Data held, once it is no longer used for the Project, unless obliged to retain the data for archival purposes in conformity with audit or legal requirements. 12. The User Institution will notify the DAC within 30 days of any changes or departures of Authorised Personnel. 13. The User Institution will notify the DAC prior to any significant changes to the protocol for the Project. 14. The User Institution will notify the DAC as soon as it becomes aware of a breach of the terms or conditions of this agreement. 15. The DAC may terminate this agreement by written notice to the User Institution. If this agreement terminates for any reason, the User Institution will be required to destroy any Data held, including copies and backup copies. This clause does not prevent the User Institution from retaining these data for archival purpose in conformity with audit or legal requirements. 16. The User Institution accepts that it may be necessary for the Data Producers to alter the terms of this agreement from time to time. In the event that changes are required, the Data Producers or their appointed agent will contact the User Institution to inform it of the changes and the User Institution may elect to accept the changes or terminate the agreement. 17. If requested, the User Institution will allow data security and management documentation to be inspected to verify that it is complying with the terms of this agreement. 18. The User Institution agrees to distribute a copy of these terms to the Authorised Personnel. The User Institution will procure that the Authorised Personnel comply with the terms of this agreement. 19. This agreement (and any dispute, controversy, proceedings or claim of whatever nature arising out of this agreement or its formation) shall be construed, interpreted and governed by the laws of the province of Ontario and PHIPA. Agreed for User Institution Signature: Name: Title: Date: Principal Investigator I confirm that I have read and understood this Agreement. Signature: Name: Title: Date: Agreed for The Data Access Committee Signature: Name: Title: Date: APPENDIX I – DATASET DETAILS APPENDIX II ––PROJECT DETAILS APPENDIX III –– PUBLICATION POLICY

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001004792 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
i
EGAF00004838203 fastq.gz 3.2 GB Report
EGAF00004838204 fastq.gz 3.2 GB Report
EGAF00004838205 fastq.gz 3.4 GB Report
EGAF00004838206 fastq.gz 3.5 GB Report
EGAF00004838207 fastq.gz 3.3 GB Report
EGAF00004838208 fastq.gz 3.4 GB Report
EGAF00004838209 fastq.gz 3.4 GB Report
EGAF00004838210 fastq.gz 3.4 GB Report
EGAF00004838211 fastq.gz 2.9 GB Report
EGAF00004838212 fastq.gz 3.0 GB Report
EGAF00004838213 fastq.gz 3.2 GB Report
EGAF00004838214 fastq.gz 3.3 GB Report
EGAF00004838215 fastq.gz 3.1 GB Report
EGAF00004838216 fastq.gz 3.2 GB Report
EGAF00004838217 fastq.gz 2.9 GB Report
EGAF00004838218 fastq.gz 3.0 GB Report
EGAF00004838219 fastq.gz 3.0 GB Report
EGAF00004838220 fastq.gz 3.0 GB Report
EGAF00004838221 fastq.gz 3.2 GB Report
EGAF00004838222 fastq.gz 3.3 GB Report
EGAF00004838223 fastq.gz 3.1 GB Report
EGAF00004838224 fastq.gz 3.2 GB Report
EGAF00004838225 fastq.gz 3.3 GB Report
EGAF00004838226 fastq.gz 3.4 GB Report
EGAF00004838227 fastq.gz 2.9 GB Report
EGAF00004838228 fastq.gz 3.0 GB Report
EGAF00004838229 fastq.gz 3.2 GB Report
EGAF00004838230 fastq.gz 3.2 GB Report
EGAF00004838231 fastq.gz 3.1 GB Report
EGAF00004838232 fastq.gz 3.2 GB Report
EGAF00004838233 fastq.gz 2.9 GB Report
EGAF00004838234 fastq.gz 3.0 GB Report
EGAF00004838235 fastq.gz 2.8 GB Report
EGAF00004838236 fastq.gz 2.9 GB Report
EGAF00004838237 fastq.gz 3.2 GB Report
EGAF00004838238 fastq.gz 3.3 GB Report
EGAF00004838239 fastq.gz 3.3 GB Report
EGAF00004838240 fastq.gz 3.4 GB Report
EGAF00004838241 fastq.gz 3.2 GB Report
EGAF00004838242 fastq.gz 3.4 GB Report
EGAF00004838243 fastq.gz 3.1 GB Report
EGAF00004838244 fastq.gz 3.2 GB Report
EGAF00004838245 fastq.gz 2.8 GB Report
EGAF00004838246 fastq.gz 2.9 GB Report
EGAF00004838247 fastq.gz 3.2 GB Report
EGAF00004838248 fastq.gz 3.3 GB Report
EGAF00004838249 fastq.gz 2.6 GB Report
EGAF00004838250 fastq.gz 2.7 GB Report
EGAF00004838251 fastq.gz 4.0 GB Report
EGAF00004838252 fastq.gz 4.2 GB Report
EGAF00004838253 fastq.gz 3.1 GB Report
EGAF00004838254 fastq.gz 3.1 GB Report
EGAF00004838255 fastq.gz 3.2 GB Report
EGAF00004838256 fastq.gz 3.3 GB Report
EGAF00004838257 fastq.gz 3.2 GB Report
EGAF00004838258 fastq.gz 3.3 GB Report
EGAF00004838259 fastq.gz 3.0 GB Report
EGAF00004838260 fastq.gz 3.1 GB Report
EGAF00004838261 fastq.gz 3.2 GB Report
EGAF00004838262 fastq.gz 3.4 GB Report
EGAF00004838263 fastq.gz 3.1 GB Report
EGAF00004838264 fastq.gz 3.2 GB Report
EGAF00004838265 fastq.gz 3.2 GB Report
EGAF00004838266 fastq.gz 3.3 GB Report
EGAF00004838267 fastq.gz 2.8 GB Report
EGAF00004838268 fastq.gz 2.9 GB Report
EGAF00004838269 fastq.gz 6.5 GB Report
EGAF00004838270 fastq.gz 6.7 GB Report
EGAF00004838271 fastq.gz 6.8 GB Report
EGAF00004838272 fastq.gz 7.1 GB Report
EGAF00004838273 fastq.gz 4.5 GB Report
EGAF00004838274 fastq.gz 4.7 GB Report
EGAF00004838275 fastq.gz 5.6 GB Report
EGAF00004838276 fastq.gz 5.9 GB Report
EGAF00004838277 fastq.gz 6.9 GB Report
EGAF00004838278 fastq.gz 7.0 GB Report
EGAF00004838279 fastq.gz 7.2 GB Report
EGAF00004838280 fastq.gz 7.3 GB Report
EGAF00004838281 fastq.gz 8.0 GB Report
EGAF00004838282 fastq.gz 8.4 GB Report
EGAF00004838283 fastq.gz 9.3 GB Report
EGAF00004838284 fastq.gz 9.6 GB Report
EGAF00004838285 fastq.gz 7.6 GB Report
EGAF00004838286 fastq.gz 7.8 GB Report
EGAF00004838287 fastq.gz 7.2 GB Report
EGAF00004838288 fastq.gz 7.4 GB Report
EGAF00004838289 fastq.gz 2.2 GB Report
EGAF00004838290 fastq.gz 2.3 GB Report
EGAF00004838291 fastq.gz 8.2 GB Report
EGAF00004838292 fastq.gz 8.1 GB Report
EGAF00004838293 fastq.gz 9.4 GB Report
EGAF00004838294 fastq.gz 9.2 GB Report
EGAF00004838295 fastq.gz 10.0 GB Report
EGAF00004838296 fastq.gz 9.9 GB Report
EGAF00004838297 fastq.gz 8.6 GB Report
EGAF00004838298 fastq.gz 8.7 GB Report
EGAF00004838299 fastq.gz 8.9 GB Report
EGAF00004838300 fastq.gz 8.7 GB Report
EGAF00004838301 fastq.gz 7.1 GB Report
EGAF00004838302 fastq.gz 7.1 GB Report
EGAF00004838303 fastq.gz 7.5 GB Report
EGAF00004838304 fastq.gz 7.5 GB Report
EGAF00004838305 fastq.gz 7.3 GB Report
EGAF00004838306 fastq.gz 7.3 GB Report
EGAF00004838307 fastq.gz 7.4 GB Report
EGAF00004838308 fastq.gz 7.3 GB Report
EGAF00004838309 fastq.gz 8.4 GB Report
EGAF00004838310 fastq.gz 8.1 GB Report
EGAF00004838311 fastq.gz 6.8 GB Report
EGAF00004838312 fastq.gz 7.0 GB Report
EGAF00004838313 fastq.gz 5.1 GB Report
EGAF00004838314 fastq.gz 5.2 GB Report
EGAF00004838315 fastq.gz 4.9 GB Report
EGAF00004838316 fastq.gz 5.1 GB Report
EGAF00004838317 fastq.gz 6.7 GB Report
EGAF00004838318 fastq.gz 6.8 GB Report
EGAF00004838319 fastq.gz 6.9 GB Report
EGAF00004838320 fastq.gz 6.8 GB Report
EGAF00004838321 fastq.gz 5.4 GB Report
EGAF00004838322 fastq.gz 5.5 GB Report
EGAF00004838323 fastq.gz 4.8 GB Report
EGAF00004838324 fastq.gz 4.8 GB Report
EGAF00004838325 fastq.gz 5.4 GB Report
EGAF00004838326 fastq.gz 5.4 GB Report
EGAF00004838327 fastq.gz 6.1 GB Report
EGAF00004838328 fastq.gz 6.0 GB Report
EGAF00004838329 fastq.gz 4.4 GB Report
EGAF00004838330 fastq.gz 4.5 GB Report
EGAF00004838331 fastq.gz 7.8 GB Report
EGAF00004838332 fastq.gz 7.8 GB Report
EGAF00004838333 fastq.gz 6.2 GB Report
EGAF00004838334 fastq.gz 6.2 GB Report
EGAF00004838335 fastq.gz 5.9 GB Report
EGAF00004838336 fastq.gz 5.9 GB Report
EGAF00004838337 fastq.gz 6.7 GB Report
EGAF00004838338 fastq.gz 6.7 GB Report
EGAF00004838339 fastq.gz 6.4 GB Report
EGAF00004838340 fastq.gz 6.4 GB Report
EGAF00004838341 fastq.gz 7.3 GB Report
EGAF00004838342 fastq.gz 7.2 GB Report
EGAF00004838343 fastq.gz 6.0 GB Report
EGAF00004838344 fastq.gz 5.9 GB Report
EGAF00004838345 fastq.gz 7.3 GB Report
EGAF00004838346 fastq.gz 7.2 GB Report
EGAF00004838347 fastq.gz 6.8 GB Report
EGAF00004838348 fastq.gz 6.8 GB Report
EGAF00004838349 fastq.gz 6.4 GB Report
EGAF00004838350 fastq.gz 6.3 GB Report
EGAF00004838351 fastq.gz 7.2 GB Report
EGAF00004838352 fastq.gz 7.1 GB Report
EGAF00004838353 fastq.gz 8.2 GB Report
EGAF00004838354 fastq.gz 8.1 GB Report
EGAF00004838355 fastq.gz 7.4 GB Report
EGAF00004838356 fastq.gz 7.3 GB Report
EGAF00004838357 fastq.gz 7.0 GB Report
EGAF00004838358 fastq.gz 7.0 GB Report
EGAF00004838359 fastq.gz 5.4 GB Report
EGAF00004838360 fastq.gz 5.5 GB Report
EGAF00004838361 fastq.gz 6.6 GB Report
EGAF00004838362 fastq.gz 6.7 GB Report
EGAF00004838363 fastq.gz 7.6 GB Report
EGAF00004838364 fastq.gz 7.6 GB Report
EGAF00004838365 fastq.gz 6.9 GB Report
EGAF00004838366 fastq.gz 7.2 GB Report
EGAF00004838367 fastq.gz 7.1 GB Report
EGAF00004838368 fastq.gz 7.4 GB Report
EGAF00004838369 fastq.gz 8.1 GB Report
EGAF00004838370 fastq.gz 8.2 GB Report
EGAF00004838371 fastq.gz 6.9 GB Report
EGAF00004838372 fastq.gz 7.1 GB Report
170 Files (924.2 GB)