DAC

UHN Genomics Data Access Committee

Dac ID Contact Person Email Access Information
EGAC00001000912 Natalie Stickle natalie [dot] stickle [at] uhn [dot] ca No additional information is available

This DAC controls 3 datasets:

Dataset ID Description Technology Samples
EGAD00001004133 Epigenetic profiling of colorectal cancer initiating cells (CC-ICs) to identify bivalently marked genes (H3K4me3 and H3K27me3 ChIP-seq), and investigation of changes in transcriptome following EZH2 inhibition using RNA-seq. Illumina HiSeq 2500,NextSeq 500 17
EGAD00001004269 This dataset includes 112 head and neck tumour samples with matched normal (blood) samples sequenced using a custom hybrid capture panel. Illumina HiSeq 2000 224
EGAD00001005140 In order to elucidate the biological pathways altered by sphingolipid modulation with N-(4-hydroxyphenyl) retinamide (4HPR) treatment in human HSPC that may contribute to the restraint in proliferation while promoting persistence of HSC self-renewal, as well as determine the mechanism of synergy in enhancement of HSC self-renewal with CB CD34+ agonists UM171 and StemRegenin 1 (SR1), we performed RNA-sequencing (RNA-Seq) of 3 pools of lin-CB cells following 2 or 4 days with DMSO, 4HPR, UM171+SR1 or 3-Factor (4HPR+UM171+SR1). We identified modulation of sphingolipid metabolism regulates self-renewal through activating coordinated stress pathways that coalesce on endoplasmic reticulum stress and autophagy programs. Illumina HiSeq 2500 25