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Combinatorial CRISPR screen identifies fitness effects of paralogues FAM50A and FAM50B

Genetic redundancy has evolved as a way for human cells to survive the loss of genes that are single copy and essential in other organisms, but also allows tumours to survive despite having highly rearranged genomes. In this study we CRISPR screen 1,191 gene pairs, including paralogues and known and predicted synthetic lethal interactions to identify 105 gene combinations whose co-disruption results in a loss of cellular fitness. 27 pairs influence fitness across multiple cell lines including the paralogues FAM50A/FAM50B, two genes of unknown function. Silencing of FAM50B occurs across a range of tumour types and in this context disruption of FAM50A reduces cellular fitness whilst promoting micronucleus formation and extensive perturbation of transcriptional programmes. This dataset includes CRISPR screening of cancer cell lines, RNA sequencing studies of cancer cell lines and also data from the sequencing of tumour xenografts collected from mice.

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001002117 Cancer Genomics

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00004911767 cram 424.5 MB
EGAF00004911768 cram 453.4 MB
EGAF00004911769 cram 460.0 MB
EGAF00004911770 cram 375.7 MB
EGAF00004911771 cram 343.4 MB
EGAF00004911772 cram 374.3 MB
EGAF00004911773 cram 404.2 MB
EGAF00004911774 cram 432.1 MB
EGAF00004911775 cram 440.8 MB
EGAF00004911776 cram 357.4 MB
EGAF00004911777 cram 327.5 MB
EGAF00004911778 cram 357.1 MB
EGAF00004911779 cram 429.3 MB
EGAF00004911780 cram 439.5 MB
EGAF00004911781 cram 397.1 MB
EGAF00004911782 cram 407.7 MB
EGAF00004911783 cram 367.9 MB
EGAF00004911784 cram 403.9 MB
EGAF00004911785 cram 438.5 MB
EGAF00004911786 cram 448.6 MB
EGAF00004911787 cram 405.3 MB
EGAF00004911788 cram 416.6 MB
EGAF00004911789 cram 376.0 MB
EGAF00004911790 cram 412.5 MB
EGAF00004911791 cram 506.4 MB
EGAF00004911792 cram 510.8 MB
EGAF00004911793 cram 463.7 MB
EGAF00004911794 cram 475.9 MB
EGAF00004911795 cram 431.3 MB
EGAF00004911796 cram 473.8 MB
EGAF00004911797 cram 543.0 MB
EGAF00004911798 cram 548.7 MB
EGAF00004911799 cram 498.1 MB
EGAF00004911800 cram 511.2 MB
EGAF00004911801 cram 463.5 MB
EGAF00004911802 cram 508.8 MB
EGAF00004911803 cram 390.8 MB
EGAF00004911804 cram 378.7 MB
EGAF00004911805 cram 416.7 MB
EGAF00004911806 cram 388.0 MB
EGAF00004911807 cram 430.9 MB
EGAF00004911808 cram 387.1 MB
EGAF00004911809 cram 393.1 MB
EGAF00004911810 cram 429.6 MB
EGAF00004911811 cram 361.8 MB
EGAF00004911812 cram 399.4 MB
EGAF00004911813 cram 386.1 MB
EGAF00004911814 cram 423.7 MB
EGAF00004911815 cram 395.2 MB
EGAF00004911816 cram 439.8 MB
EGAF00004911817 cram 394.5 MB
EGAF00004911818 cram 401.8 MB
EGAF00004911819 cram 436.8 MB
EGAF00004911820 cram 370.0 MB
EGAF00004911821 cram 385.6 MB
EGAF00004911822 cram 377.2 MB
EGAF00004911823 cram 400.0 MB
EGAF00004911824 cram 384.8 MB
EGAF00004911825 cram 427.5 MB
EGAF00004911826 cram 379.7 MB
EGAF00004911827 cram 385.2 MB
EGAF00004911828 cram 422.3 MB
EGAF00004911829 cram 357.3 MB
EGAF00004911830 cram 392.9 MB
EGAF00004911831 cram 382.8 MB
EGAF00004911832 cram 406.5 MB
EGAF00004911833 cram 390.5 MB
EGAF00004911834 cram 434.3 MB
EGAF00004911835 cram 385.6 MB
EGAF00004911836 cram 392.2 MB
EGAF00004911837 cram 426.0 MB
EGAF00004911838 cram 364.4 MB
EGAF00004911839 cram 372.6 MB
EGAF00004911840 cram 364.3 MB
EGAF00004911841 cram 383.4 MB
EGAF00004911842 cram 370.5 MB
EGAF00004911843 cram 412.0 MB
EGAF00004911844 cram 367.7 MB
EGAF00004911845 cram 374.5 MB
EGAF00004911846 cram 406.9 MB
EGAF00004911847 cram 346.2 MB
EGAF00004911848 cram 379.5 MB
EGAF00004911849 cram 370.1 MB
EGAF00004911850 cram 387.8 MB
EGAF00004911851 cram 376.5 MB
EGAF00004911852 cram 419.1 MB
EGAF00004911853 cram 373.4 MB
EGAF00004911854 cram 380.9 MB
EGAF00004911855 cram 412.7 MB
EGAF00004911856 cram 352.7 MB
EGAF00004911857 cram 394.2 MB
EGAF00004911858 cram 386.8 MB
EGAF00004911859 cram 397.0 MB
EGAF00004911860 cram 393.4 MB
EGAF00004911861 cram 433.7 MB
EGAF00004911862 cram 388.6 MB
EGAF00004911863 cram 393.1 MB
EGAF00004911864 cram 434.4 MB
EGAF00004911865 cram 367.7 MB
EGAF00004911866 cram 411.9 MB
EGAF00004911867 cram 403.3 MB
EGAF00004911868 cram 404.9 MB
EGAF00004911869 cram 410.5 MB
EGAF00004911870 cram 452.2 MB
EGAF00004911871 cram 403.4 MB
EGAF00004911872 cram 408.8 MB
EGAF00004911873 cram 452.2 MB
EGAF00004911874 cram 384.1 MB
EGAF00004911875 cram 401.5 MB
EGAF00004911876 cram 391.3 MB
EGAF00004911877 cram 412.8 MB
EGAF00004911878 cram 396.4 MB
EGAF00004911879 cram 440.5 MB
EGAF00004911880 cram 392.9 MB
EGAF00004911881 cram 398.8 MB
EGAF00004911882 cram 437.7 MB
EGAF00004911883 cram 373.6 MB
EGAF00004911884 cram 392.7 MB
EGAF00004911885 cram 383.3 MB
EGAF00004911886 cram 406.1 MB
EGAF00004911887 cram 388.3 MB
EGAF00004911888 cram 432.8 MB
EGAF00004911889 cram 385.0 MB
EGAF00004911890 cram 390.2 MB
EGAF00004911891 cram 430.7 MB
EGAF00004911892 cram 364.6 MB
EGAF00004911893 cram 401.0 MB
EGAF00004911894 cram 393.0 MB
EGAF00004911895 cram 399.4 MB
EGAF00004911896 cram 403.0 MB
EGAF00004911897 cram 445.9 MB
EGAF00004911898 cram 392.1 MB
EGAF00004911899 cram 395.7 MB
EGAF00004911900 cram 438.0 MB
EGAF00004911901 cram 373.5 MB
EGAF00004911902 cram 390.9 MB
EGAF00004911903 cram 381.5 MB
EGAF00004911904 cram 383.2 MB
EGAF00004911905 cram 391.6 MB
EGAF00004911906 cram 433.4 MB
EGAF00004911907 cram 379.5 MB
EGAF00004911908 cram 385.5 MB
EGAF00004911909 cram 424.5 MB
EGAF00004911910 cram 364.4 MB
EGAF00004911911 cram 737.7 MB
EGAF00004911912 cram 727.7 MB
EGAF00004911913 cram 707.7 MB
EGAF00004911914 cram 737.7 MB
EGAF00004911915 cram 664.6 MB
EGAF00004911916 cram 702.1 MB
EGAF00004911917 cram 774.6 MB
EGAF00004911918 cram 765.3 MB
EGAF00004911919 cram 745.0 MB
EGAF00004911920 cram 777.2 MB
EGAF00004911921 cram 699.1 MB
EGAF00004911922 cram 734.8 MB
EGAF00004911923 cram 726.6 MB
EGAF00004911924 cram 714.8 MB
EGAF00004911925 cram 694.8 MB
EGAF00004911926 cram 724.7 MB
EGAF00004911927 cram 654.1 MB
EGAF00004911928 cram 683.0 MB
EGAF00004911929 cram 796.1 MB
EGAF00004911930 cram 784.5 MB
EGAF00004911931 cram 763.9 MB
EGAF00004911932 cram 796.0 MB
EGAF00004911933 cram 718.2 MB
EGAF00004911934 cram 746.6 MB
EGAF00004911935 cram 788.7 MB
EGAF00004911936 cram 777.5 MB
EGAF00004911937 cram 758.6 MB
EGAF00004911938 cram 781.8 MB
EGAF00004911939 cram 709.2 MB
EGAF00004911940 cram 731.3 MB
EGAF00004911941 cram 787.8 MB
EGAF00004911942 cram 774.7 MB
EGAF00004911943 cram 756.6 MB
EGAF00004911944 cram 783.3 MB
EGAF00004911945 cram 708.7 MB
EGAF00004911946 cram 733.5 MB
EGAF00004911947 cram 731.4 MB
EGAF00004911948 cram 784.1 MB
EGAF00004911949 cram 704.6 MB
EGAF00004911950 cram 792.2 MB
EGAF00004911951 cram 690.4 MB
EGAF00004911952 cram 718.6 MB
EGAF00004911953 cram 734.4 MB
EGAF00004911954 cram 786.8 MB
EGAF00004911955 cram 704.6 MB
EGAF00004911956 cram 794.9 MB
EGAF00004911957 cram 692.2 MB
EGAF00004911958 cram 720.1 MB
EGAF00004911959 cram 829.0 MB
EGAF00004911960 cram 889.8 MB
EGAF00004911961 cram 742.3 MB
EGAF00004911962 cram 921.5 MB
EGAF00004911963 cram 762.2 MB
EGAF00004911964 cram 805.1 MB
EGAF00004911965 cram 823.5 MB
EGAF00004911966 cram 883.5 MB
EGAF00004911967 cram 740.0 MB
EGAF00004911968 cram 914.7 MB
EGAF00004911969 cram 757.5 MB
EGAF00004911970 cram 798.9 MB
EGAF00004911971 cram 786.8 MB
EGAF00004911972 cram 842.8 MB
EGAF00004911973 cram 745.2 MB
EGAF00004911974 cram 856.7 MB
EGAF00004911975 cram 733.6 MB
EGAF00004911976 cram 767.2 MB
EGAF00004911977 cram 771.5 MB
EGAF00004911978 cram 826.6 MB
EGAF00004911979 cram 737.3 MB
EGAF00004911980 cram 841.7 MB
EGAF00004911981 cram 724.4 MB
EGAF00004911982 cram 754.8 MB
216 Files (113.3 GB)