ADT/SPEX CITE-Seq performance dataset

Dataset ID Technology Samples
EGAD00001008419 unspecified 10

Dataset Description

To understand the impact of enzymatic treatments on gene expression and epitope preservation on major immune cell populations, skin dissociation (SkinD) and solid soft tumor dissociation (TumorD) were tested on three healthy PBMC samples in triplicate (D1, D2, D3), against an untreated control.
CITE-seq performance was assessed on a solid biopsy cohort of 11 samples (5 healthy skin samples, 3 primary melanoma samples, 3 melanoma metastasis samples) as well as on a liquid biopsy PBMC cohort consisting of three healthy donors and three immunotherapy-treated melanoma patients.
This dataset contains the ADT/SPEX data for each sample.
Data is provided in the form of pooled BAM files. Linkage between samples, BAM files and hashtags is provided in a separate linkage file.

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

Cite-seq committee
Contact person: Dr. Mitchell Levesque
Email: mithchell [dot] levesque [at] usz [dot] ch
More details: EGAC00001002453


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