Dataset

LLDeep DAG2+ scRNA-seq in PBMCs

Dataset ID Technology Samples
EGAD00010001315 Illumina HiSeq4000 45

Dataset Description

Single cell transcriptomics of PBMCs of 47 donors from the Lifelines Deep cohort (general population, Northern part of the Netherlands). Cells of five or six different donors were pooled together in one sample pool, resulting in eight different sample pools. In total, 28.855 cells were captured and their transcriptomes were sequenced to an average depth of 74k. Genotype data was available for each donor, which allowed us to use the Demuxlet method that uses variable SNPs between the pooled individuals to determine which cell belongs to which individual. Since genotype information is lacking of 2 individuals, the transcriptome of only 45 individuals could be retrieved.

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

LLDEEP DAG2+ Data Access Committee
Contact person: Jackie Dekens
Email: j [dot] a [dot] m [dot] dekens [at] umcg [dot] nl
More details: EGAC00001000694

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