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Biokey PBMC: 10x Chromium single-cell RNA sequencing

Single-cell RNA sequencing of serial peripheral blood mononuclear cell (PBMC) samples (n=76), collected at baseline (Pre) and after 9 days of anti-PD1 treatment (On). Of these, 68 PBMC samples were pooled in 34 pools of two, loaded per lane on the 10x Genomics chip in equal proportions according to a pre-designed pooling matrix. Patients were bioinformatically demultiplexed using Souporcell (Heaton et al., Nature Methods, 2020). A metadata file detailing which barcode originated from which patient is included.

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VIB Policy for Data Access and Data Transfer

Applicability This policy applies to the access to and transfer of VIB research datasets that contain non-anonymous Personal Data which are subject to the provisions of the General Data Protection Regulation (EU) 2016/679. Access/transfer conditions - The access/transfer is subject to approval by the VIB Data Access Committee. - The proposed research purpose should not be incompatible with the primary research purpose for which the data were originally generated. - The Recipient shall have in place adequate safeguards for the protection of Personal Data. - Access is subject to a Data Transfer/Access Agreement which will be managed by VIB Innovation & Business. - The Data Transfer/Access will be limited to the Data that are necessary for the proposed research. Access/transfer procedure - Requests to access or obtain non-anonymous datasets should be submitted within the EGA portal, which will then generate a notification to the responsible corresponding author that has uploaded the dataset into the repository, and with a copy to dac@vib.be. - VIB will send a request form to the applicant to complete. - The completed form should amongst other request should contain the following information: o Name, affiliation (legal entity) and contact information of the requesting recipient party. o Reference to the specific dataset(s) for which access or transfer is requested. o Description of the research purpose for which the data will be used. - The VIB Data Access Committee takes decisions to approve access/transfer within 14 days upon receipt of the completed request form. - Data access will be given / Data will be transferred after completion of the Data Transfer/Access Agreement.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000002113 Transcriptome Sequencing

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF50000975504 fastq.gz 2.1 GB
EGAF50000975505 fastq.gz 4.8 GB
EGAF50000975506 fastq.gz 3.0 GB
EGAF50000975507 fastq.gz 7.3 GB
EGAF50000975508 fastq.gz 1.2 GB
EGAF50000975509 fastq.gz 2.8 GB
EGAF50000975510 fastq.gz 1.3 GB
EGAF50000975511 fastq.gz 3.1 GB
EGAF50000975512 fastq.gz 2.6 GB
EGAF50000975513 fastq.gz 6.3 GB
EGAF50000975514 fastq.gz 1.1 GB
EGAF50000975515 fastq.gz 2.6 GB
EGAF50000975516 fastq.gz 5.6 GB
EGAF50000975517 fastq.gz 13.7 GB
EGAF50000975518 fastq.gz 4.1 GB
EGAF50000975519 fastq.gz 10.1 GB
EGAF50000975520 fastq.gz 2.3 GB
EGAF50000975521 fastq.gz 5.4 GB
EGAF50000975522 fastq.gz 2.5 GB
EGAF50000975523 fastq.gz 5.9 GB
EGAF50000975524 fastq.gz 2.1 GB
EGAF50000975525 fastq.gz 5.0 GB
EGAF50000975526 fastq.gz 2.7 GB
EGAF50000975527 fastq.gz 6.5 GB
EGAF50000975528 fastq.gz 2.9 GB
EGAF50000975529 fastq.gz 6.9 GB
EGAF50000975530 fastq.gz 771.7 MB
EGAF50000975531 fastq.gz 1.6 GB
EGAF50000975532 fastq.gz 4.4 GB
EGAF50000975533 fastq.gz 10.0 GB
EGAF50000975534 fastq.gz 2.4 GB
EGAF50000975535 fastq.gz 5.9 GB
EGAF50000975536 fastq.gz 731.0 MB
EGAF50000975537 fastq.gz 1.5 GB
EGAF50000975538 fastq.gz 4.5 GB
EGAF50000975539 fastq.gz 9.4 GB
EGAF50000975540 fastq.gz 2.6 GB
EGAF50000975541 fastq.gz 6.3 GB
EGAF50000975542 fastq.gz 2.5 GB
EGAF50000975543 fastq.gz 6.0 GB
EGAF50000975544 fastq.gz 790.4 MB
EGAF50000975545 fastq.gz 1.5 GB
EGAF50000975546 fastq.gz 1.8 GB
EGAF50000975547 fastq.gz 4.3 GB
EGAF50000975548 fastq.gz 1.3 GB
EGAF50000975549 fastq.gz 3.2 GB
EGAF50000975550 fastq.gz 2.0 GB
EGAF50000975551 fastq.gz 4.8 GB
EGAF50000975552 fastq.gz 2.7 GB
EGAF50000975553 fastq.gz 6.6 GB
EGAF50000975554 fastq.gz 3.2 GB
EGAF50000975555 fastq.gz 7.8 GB
EGAF50000975556 fastq.gz 935.5 MB
EGAF50000975557 fastq.gz 2.0 GB
EGAF50000975558 fastq.gz 1.3 GB
EGAF50000975559 fastq.gz 2.7 GB
EGAF50000975560 fastq.gz 3.6 GB
EGAF50000975561 fastq.gz 8.6 GB
EGAF50000975562 fastq.gz 3.6 GB
EGAF50000975563 fastq.gz 8.6 GB
EGAF50000975564 fastq.gz 1.6 GB
EGAF50000975565 fastq.gz 3.7 GB
EGAF50000975566 fastq.gz 3.9 GB
EGAF50000975567 fastq.gz 9.3 GB
EGAF50000975568 fastq.gz 3.1 GB
EGAF50000975569 fastq.gz 7.5 GB
EGAF50000975570 fastq.gz 3.7 GB
EGAF50000975571 fastq.gz 9.1 GB
EGAF50000975572 fastq.gz 3.7 GB
EGAF50000975573 fastq.gz 9.1 GB
EGAF50000975574 fastq.gz 4.3 GB
EGAF50000975575 fastq.gz 10.3 GB
EGAF50000975576 fastq.gz 4.6 GB
EGAF50000975577 fastq.gz 11.8 GB
EGAF50000975578 fastq.gz 2.4 GB
EGAF50000975579 fastq.gz 5.7 GB
EGAF50000975580 fastq.gz 5.2 GB
EGAF50000975581 fastq.gz 12.6 GB
EGAF50000975582 fastq.gz 3.3 GB
EGAF50000975583 fastq.gz 8.0 GB
EGAF50000975584 fastq.gz 2.3 GB
EGAF50000975585 fastq.gz 5.5 GB
EGAF50000975586 fastq.gz 2.4 GB
EGAF50000975587 fastq.gz 5.8 GB
EGAF50000975672 csv 32.5 MB
85 Files (382.7 GB)