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VIB Institutional DAC

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VIB-UZL Data Access Committee
dac@vib.be

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This DAC controls 5 datasets

Dataset ID	Description	Technology	Samples
EGAD00001009686	This dataset contains both snRNA-seq and bulk RNA-seq data from 19 different patients, comprising of 9 healthy controls (4 Spinal Cord, 5 Motor Cortex) as well as 5 C9ALS patients and 5 sporadic ALS patients, each with paired data from the spinal cord and motor cortex. For the snRNA-seq data, fastq files containing the raw reads are provided, many of these samples were pooled for sequencing and subsequently require demultiplexing using SNPs using a tool such as freemuxlet. The paired bulk RNA-seq data (raw fastq files) can be used to acquire the ground truth per patient - a list of pooled samples can be found in map.txt. snRNA-seq data was produced using the 10X Genomics 3' v3 kit and bulk RNA-seq was produced using the Illumina TruSeq v2 kit.	Illumina NovaSeq 6000	64
EGAD50000000378	FFPE tissue and liquid biopsy (blood, pleural and peritoneal effusions) samples, processed with the cfRRBS (cell-free reduced representation bisulfite sequencing) protocol.	unspecified	181
EGAD50000000389	This dataset contains 228 paired fastq files sequenced with Illumina Novaseq 6000, and the file with the sample and clinical data.	Illumina NovaSeq 6000	114
EGAD50000000553	cfRRBS data produced from the cfDNA in plasma isolated from healthy, adult donors.	Illumina NovaSeq 6000	44
EGAD50000000554	In this study, we explore the potential of classifying pediatric brain tumors based on methylation profiling of the cell-free DNA in cerebrospinal fluid (CSF). For this proof-of-concept study, we collected 20 cerebrospinal fluid samples of pediatric brain cancer patients via a ventricular drain placed for reasons of increased intracranial pressure. For 11 patients in this study we collected matched tumor DNA. This cohort contains fastQ files of cfRRBS data of these samples.	Illumina NovaSeq 6000	43