15394 results for "1 mil coins fc 26 Visit Buyfc26coins.com for latest FC 26 coins news..buoc"

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• A95623A

  Whole genome sequencing for single cells for library A95623A 1897 cells; filetype=bam

  Dataset EGAD00001007607

• A95668B

  Whole genome sequencing for single cells for library A95668B 1905 cells; filetype=bam

  Dataset EGAD00001007609

• A95720A

  Whole genome sequencing for single cells for library A95720A 1467 cells; filetype=bam

  Dataset EGAD00001007613

• A96190A

  Whole genome sequencing for single cells for library A96190A 1833 cells; filetype=bam

  Dataset EGAD00001007620

• A96233B

  Whole genome sequencing for single cells for library A96233B 1601 cells; filetype=bam

  Dataset EGAD00001007623

• A98234B

  Whole genome sequencing for single cells for library A98234B 1600 cells; filetype=bam

  Dataset EGAD00001007627

• A98304A

  Whole genome sequencing for single cells for library A98304A 1560 cells; filetype=bam

  Dataset EGAD00001007636

• A108851B

  Whole genome sequencing for single cells for library A108851B 1681 cells; filetype=bam

  Dataset EGAD00001007596

• A108870A

  Whole genome sequencing for single cells for library A108870A 1795 cells; filetype=bam

  Dataset EGAD00001007598

• Targeted de novo phasing and long-range assembly by template mutagenesis

  Long-range sequencing with low error rate has been challenging. Sequence assembly and phasing usually require a high-quality reference genome for mapping, so working on highly-variable genomic regions or regions with no reference genome information would be difficult. In this study, we describe novel bench protocols and algorithms to obtain ultra-low-error-rate haplotype-phased sequence assemblies of regions 10 KB in length using a short-read sequencing platform that simultaneously solves the above two problems. We accomplish this by imprinting each template strand from a target region with a dense and unique mutation pattern. The mutation process randomly and independently converts ~50% of cytosines to uracils. Short-read sequencing libraries are made from both mutated and unmutated templates. A conservative de Bruijn graph approach seeds an assembly of the mutated templates, which we then extend by mapping paired-end reads. We next partition the template assemblies into two or more haplotypes after using the unmutated sequence library to recover almost all of the mutated bases. The final haplotype is assembled and corrected for residual template mutations and PCR errors. We obtain per-base-error rates below 10 9. We apply this method to a human family, correctly assembling and phasing three genomic intervals, including the highly polymorphic HLA-B gene.

  Dataset EGAD00001008444

• 59 CLPD-NK cases WGS & WTS data

  For the cohort of 59 samples, we performed TruSeq DNA PCR-Free whole-genome sequencing library preparation according to manufacturer’s instructions (llumina, ILMN, San Diego, CA) on the automated NGS Star liquid handling platform (Hamilton, Bonaduz, Switzerland) followed by 2x150 bp paired-end sequencing on the HiSeqX or NovaSeq6000 (ILMN). An average coverage of >100x was achieved. For whole transcriptome analysis, the TruSeq Total Stranded RNA kit was used, starting with 250 ng of total RNA, to generate RNA libraries following the manufacturer’s recommendations (ILMN). 2x100bp paired-end reads were sequenced on the NovaSeq 6000 with a median of 50 mio. reads per sample (ILMN).

  Dataset EGAD00001008558

• A95724B

  Whole genome sequencing for single cells for library A95724B 581 samples; filetype=bam

  Dataset EGAD00001008230

• A96172A

  Whole genome sequencing for single cells for library A96172A 1476 samples; filetype=bam

  Dataset EGAD00001008240

• A95724A

  Whole genome sequencing for single cells for library A95724A 503 samples; filetype=bam

  Dataset EGAD00001008229

• A96146A

  Whole genome sequencing for single cells for library A96146A 1195 samples; filetype=bam

  Dataset EGAD00001008233

• A96149B

  Whole genome sequencing for single cells for library A96149B 1792 samples; filetype=bam

  Dataset EGAD00001008235

• A96162B

  Whole genome sequencing for single cells for library A96162B 1316 samples; filetype=bam

  Dataset EGAD00001008238

• A96172B

  Whole genome sequencing for single cells for library A96172B 1694 samples; filetype=bam

  Dataset EGAD00001008241

• A96175C

  Whole genome sequencing for single cells for library A96175C 1473 samples; filetype=bam

  Dataset EGAD00001008243

• A96179B

  Whole genome sequencing for single cells for library A96179B 1396 samples; filetype=bam

  Dataset EGAD00001008245

• A96180B

  Whole genome sequencing for single cells for library A96180B 1189 samples; filetype=bam

  Dataset EGAD00001008246

• A96183C

  Whole genome sequencing for single cells for library A96183C 1036 samples; filetype=bam

  Dataset EGAD00001008247

• A96184A

  Whole genome sequencing for single cells for library A96184A 1733 samples; filetype=bam

  Dataset EGAD00001008248

• A96186A

  Whole genome sequencing for single cells for library A96186A 850 samples; filetype=bam

  Dataset EGAD00001008249

• A96186C

  Whole genome sequencing for single cells for library A96186C 525 samples; filetype=bam

  Dataset EGAD00001008250