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Long-read and short-read isoform sequencing in breast cancer

The goal of this project was to perform long-read RNA sequencing (LR-seq, PacBio) in combination with short-read RNA-seq for systematic characterization of the isoform diversity in primary breast tumor samples. We sequenced the full-length transcriptomes of 26 breast tumors and 4 normal breast samples.

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID Description Technology Samples
EGAD00001006596 NextSeq 500 25
EGAD00001006597 PacBio RS II Sequel 26
EGAD00001009514 NextSeq 500 PacBio RS II Sequel 30
Publications Citations
A comprehensive long-read isoform analysis platform and sequencing resource for breast cancer.
Sci Adv 8: 2022 eabg6711
A widespread length-dependent splicing dysregulation in cancer.
Sci Adv 8: 2022 eabn9232