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Epigenomic atlas of organoid development

To investigfate the epigenomic basis of fate decisions in the central nerveous system, we recorded a epigenomic timecourse of human brain organoid development using Cut&Tag with paired scRNA-seq. We further performed perturbation experiment inhibiting EED and profiled the response with scRNA-seq and Cut&Tag. Finally, we profiled a sample of the developing human brain using multiome sequencing and Cut&Tag. In our study, we uncover epigenomic switches during brain region diversification and show that epigenetic activation precedes gene expression during neurogenesis in organoids and in primary tissue. Lastly, we show that transcriptional repression by H3K27me3 is required to stabilize neuroectoderm induction from pluripotency.

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID Description Technology Samples
EGAD50000000222 unspecified 8
EGAD50000000223 unspecified 8
EGAD50000000224 unspecified 2
EGAD50000000225 unspecified 1
Publications Citations
Single-cell epigenomic reconstruction of developmental trajectories from pluripotency in human neural organoid systems.
Nat Neurosci 27: 2024 1376-1386
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